RNA isolation from recalcitrant plant tissue
| العنوان: | RNA isolation from recalcitrant plant tissue |
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| المؤلفون: | June I. Medford, Diana Cox-Foster, Ralph O. Mumma, Richard Craig, David J. Schultz |
| المصدر: | Plant Molecular Biology Reporter. 12:310-316 |
| بيانات النشر: | Springer Science and Business Media LLC, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | Euphorbia, biology, Pedicel, Geranium, Botany, RNA, Plant Science, RNA extraction, Pelargonium, biology.organism_classification, Molecular Biology, Poinsettia, Trichome |
| الوصف: | The isolation of high-quality RNA from various tissues (leaves, pedicels, glandular trichomes) of garden geranium (Pelargonium xhortorum) using various published methods is difficult due to numerous oxidizing compounds. A new RNA extraction method was developed through the combination and modification of two separate procedures (Rochester et al., 1986; Manning 1991). In addition to geranium tissues, this method is successful when used with other recalcitrant tissues such as mature needles of white pine (Pinus strobus) and mature leaves of poinsettia (Euphorbia pulcherrima). RNA quality was judged by spectrophotometric readings, denaturing agarose gels, and successful reverse transcription. |
| تدمد: | 1572-9818 0735-9640 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::a23784510021aa75fd7d4877475b658b https://doi.org/10.1007/bf02669273 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........a23784510021aa75fd7d4877475b658b |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15729818 07359640 |
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