Background The aim was to assess iron status and dietary iron intake in a sample of premenopausal female regular and new blood donors. Study Design and Methods Premenopausal women blood donors were invited to participate. Blood samples were analyzed for serum ferritin and hemoglobin. An iron checklist assessed dietary iron intake. Donors were classified as regular donors or new donors. Results Twenty-one new donors (mean [SD] age, 28.6 [6.0] years; body mass index [BMI], 25.6 [4.5] kg/m2) and 172 regular donors (mean age, 29.4 [5.5] years; BMI, 24.7 [3.8] kg/m2) participated. Fifty percent of regular donors and 24% of new donors had depleted iron stores (serum ferritin