Separation of Heparin into Subtractions by DEAE-Cellulose Chromatography
| العنوان: | Separation of Heparin into Subtractions by DEAE-Cellulose Chromatography |
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| المؤلفون: | Toby Starr, Menard M. Gertler, Robert H. Yue |
| المصدر: | Thrombosis and Haemostasis. 42:1452-1459 |
| بيانات النشر: | Georg Thieme Verlag KG, 1979. |
| سنة النشر: | 1979 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Chromatography, Antithrombin, Low activity, Salt (chemistry), Hematology, Uronic acid, Heparin, chemistry.chemical_compound, Thrombin, chemistry, medicine, High activity, DEAE-Cellulose Chromatography, medicine.drug |
| الوصف: | SummaryCommercial porcine heparin can be separated into three distinct subtractions by using DEAE-cellulose chromatography and a stepped salt gradient. Gram quantities of heparin can be fractionated by this technique. All three heparin subtractions can accelerate the inhibition of thrombin by antithrombin III with different efficiency. The specific activities of the high activity heparin, intermediate activity heparin and low activity heparin are 228 units/mg, 142 units/mg and 95 units/mg, respectively. Both the uronic acid content and the quantity of N-SO4 for all three heparin subfractions have been evaluated. The high activity heparin has the lowest uronic acid and N-SO4 content. The successful separation of commercial heparin into three distinct subfractions by means of ion-exchange chromatography suggests that the net charge on these three heparin components will serve as a model system in the elucidation of the structure and activity relationship to the biological function of heparin. |
| تدمد: | 2567-689X 0340-6245 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::a537bb6e918eaa5266b9a186c210e428 https://doi.org/10.1055/s-0038-1657046 |
| رقم الأكسشن: | edsair.doi...........a537bb6e918eaa5266b9a186c210e428 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2567689X 03406245 |
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