Meta-analysis of lean and obese RNA-seq datasets to identify genes targeting obesity
| العنوان: | Meta-analysis of lean and obese RNA-seq datasets to identify genes targeting obesity |
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| المؤلفون: | Lavanya Prabhakar |
| المصدر: | Bioinformation. 19:331-335 |
| بيانات النشر: | Biomedical Informatics, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | General Medicine |
| الوصف: | Obesity is a global crisis leading to several metabolic disorders. Modernization and technology innovation has been easier for next generation sequencing using open-source online software galaxy, which allows the users to share their data and workflow mapping in an effortless manner. This study is to identify candidate genes for obesity by performing differential expression of genes. RNA-Seq analysis was performed for six different datasets retrieved from GEO database. 258 datasets from obese patients and 55 datasets from lean patients were analysed for differentially expressed genes (DEGs). DEGs analysis showed 1971 upregulated genes and 615 downregulated genes with log2FC count ≥ 2.5 and p-value < 0.05. The Gene enrichment analysis performed using Gene Ontology resource highlighted pathways associated to obesity such as cholesterol metabolism, Fat digestion and absorption and glycerolipid metabolism. Using string database protein-protein interactions network was built and the network clusters were visualized using Cytoscape software. The protein-protein interactions of the upregulated and downregulated genes were mapped to form a network, wherein PNLIP (Pancreatic lipase) and FTO (Fat mass and obesity associated protein) gene clusters were visualized as densely connected clusters in MCODE. PNLIP and FTO with its associated genes were identified as candidate genes for targeting obesity. |
| تدمد: | 0973-2063 0973-8894 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::a62beac2bca5edbc6f2a6ba159c0f392 https://doi.org/10.6026/97320630019331 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........a62beac2bca5edbc6f2a6ba159c0f392 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09732063 09738894 |
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