Lysophosphatidylinositol‐acyltransferase‐1 is involved in cytosolic Ca2+oscillations in macrophages
| العنوان: | Lysophosphatidylinositol‐acyltransferase‐1 is involved in cytosolic Ca2+oscillations in macrophages |
|---|---|
| المؤلفون: | Masaaki Ito, Hiroki Nakanishi, Kiyomi Nigorikawa, Junko Sasaki, Shinya Takemasu, Shunsuke Takasuga, Kaoru Hazeki, Shin Morioka, Isao Matsuoka, Takehiko Sasaki, Satoshi Kofuji, Satoshi Eguchi |
| المصدر: | Genes to Cells. 24:366-376 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0303 health sciences, Inflammation, Stimulation, Cell Biology, Biology, Phospholipase, Cell biology, 03 medical and health sciences, Cytosol, Immune system, Genetics, medicine, Extracellular, medicine.symptom, Receptor, Intracellular, 030304 developmental biology |
| الوصف: | Lysophosphatidylinositol-acyltransferase-1 (LPIAT1) specifically catalyzes the transfer of arachidonoyl-CoA to lysophosphoinositides. LPIAT-/- mice have been shown to have severe defects in the brain and liver; however, the exact molecular mechanisms behind these conditions are not well understood. As immune cells have been implicated in liver inflammation based on disfunction of LPIAT1, we generated Raw264.7 macrophages deficient in LPIAT1, using shRNA and CRISPR/Cas9. The amount of C38:4 species in phosphoinositides, especially in PtdInsP2 , was remarkably decreased in these cells. Unlike in wild-type cells, LPIAT1-deficient cells showed prolonged oscillations of intracellular Ca2+ upon UDP stimulation, which is known to activate phospholipase Cβ through the Gq-coupled P2Y6 receptor, even in the absence of extracellular Ca2+ . It is speculated that the prolonged Ca2+ response may be relevant to the increased risk of liver inflammation induced by LPIAT1 disfunction. |
| تدمد: | 1365-2443 1356-9597 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::a88d4009f2130e2e2dbc3f8f0c1beedb https://doi.org/10.1111/gtc.12681 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........a88d4009f2130e2e2dbc3f8f0c1beedb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652443 13569597 |
|---|