Key genes for phenylpropanoid metabolite biosynthesis during half-highbush blueberry (Vaccinium angustifolium×Vaccinium corymbosum) fruit development
| العنوان: | Key genes for phenylpropanoid metabolite biosynthesis during half-highbush blueberry (Vaccinium angustifolium×Vaccinium corymbosum) fruit development |
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| المؤلفون: | Bofei Yang, Yan Song, Yuening Li, Xianglong Wang, Qingxun Guo, Lianxia Zhou, Yang Zhang, Chunyu Zhang |
| المصدر: | Journal of Berry Research. 12:297-311 |
| بيانات النشر: | IOS Press, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | food and beverages, Soil Science, Plant Science, Horticulture, complex mixtures, Agronomy and Crop Science, Biochemistry, Food Science |
| الوصف: | BACKGROUND: Blueberry fruits contain large amounts of phenolic compounds derived from the phenylpropanoid pathway. Their biosynthesis is complex, involving many enzymes. OBJECTIVE: We sought to investigate the content of phenylpropanoid-derived compounds and identify key genes involved in the phenylpropanoid metabolite pathway during half-highbush blueberry fruit development. METHODS: Phenylpropanoid metabolite contents were determined by high-performance liquid chromatography (HPLC) and spectrophotometry. Gene expression was examined through reverse-transcription PCR. RESULTS: Phloretin, chlorogenic acid, total flavonol, quercetin, catechin, and proanthocyanidin contents were highest in small-sized green fruits; myricetin and epicatechin contents were highest in pink fruits; and lignin and anthocyanin were highest in blue fruits. Genes from the 4-coumarate CoA ligase (4CL) family regulate the biosynthesis of phenylpropanoid metabolites. Phenylalanine ammonia-lyase (PAL) and cinnamoyl-CoA reductase (CCR) are key genes in the lignin biosynthetic pathway. Flavonol synthase (FLS) is a key gene affecting total flavonols and the quercetin biosynthetic pathway. PAL and chalcone isomerase (CHI) are key genes in the epicatechin and anthocyanin biosynthetic pathways, respectively. CONCLUSION: Phenylpropanoid metabolites are regulated by multiple genes from the same or different families. Enzymes in different metabolic pathways compete for precursors to form a complex regulatory network for phenylalanine metabolism. |
| تدمد: | 1878-5123 1878-5093 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::a968b3905c3e96862e32426bca5c711e https://doi.org/10.3233/jbr-211554 |
| رقم الأكسشن: | edsair.doi...........a968b3905c3e96862e32426bca5c711e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18785123 18785093 |
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