Rapid detection of chitosanase activity in chitosanase gene-transformed strains of Enterobacter cloacae by lytic infection of specific bacteriophages
| العنوان: | Rapid detection of chitosanase activity in chitosanase gene-transformed strains of Enterobacter cloacae by lytic infection of specific bacteriophages |
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| المؤلفون: | Yuuki Ishii, Hideyoshi Toyoda, Koji Kakutani, Teruo Nonomura, Shigeyuki Mayama, Yoshinori Matsuda, Yukio Tosa, Keiichi Fujiwara, Yoshihiro Takikawa |
| المصدر: | Journal of General Plant Pathology. 69:131-137 |
| بيانات النشر: | Springer Science and Business Media LLC, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Agar plate, Lysis, Chitosanase activity, biology, Strain (chemistry), Lytic cycle, Virulence, Plant Science, Chitosanase, biology.organism_classification, Agronomy and Crop Science, Enterobacter cloacae, Microbiology |
| الوصف: | In combination with lytic infection by virulent phages, a simple method for monitoring transgenic strains of Enterobacter cloacae was developed in this study. First, 15 strains of E. cloacae were used as indicator bacteria to isolate virulent phages with different host ranges. Of the phages isolated, five isolates (EcP-22, -35, -45, -55, and -70) were used to construct a set of virulent phages corresponding to all strains of E. cloacae. Using this phage set, a rhizosphere strain (KRM-055E) of E. cloacae was effectively screened from field soil. KRM-055E was transformed with a prokaryotic chitosanase gene csnSM1 and infected with the phage EcP-03, which can lyse the strain most effectively. The lysis of KRM-055E/csn occurred 2 h after inoculation, and the chitosanase activity was simply detected by dropping the lysate onto an agar plate containing glycol chitosan. The positive signal for chitosanase activity was detected in the 2-h lysates, and the signal intensity reached a maximum in the 5-h lysate. The present assay was simple, rapid, inexpensive, easy to perform, and applicable to another strains. |
| تدمد: | 1610-739X 1345-2630 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::a9fc69c2e4b7e2d7a9c747d826d6314f https://doi.org/10.1007/s10327-002-0025-8 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........a9fc69c2e4b7e2d7a9c747d826d6314f |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 1610739X 13452630 |
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