Cytotoxicity and genotoxicity of silver nanoparticles in Chinese Hamster ovary cell line (CHO-K1) cells
| العنوان: | Cytotoxicity and genotoxicity of silver nanoparticles in Chinese Hamster ovary cell line (CHO-K1) cells |
|---|---|
| المؤلفون: | Seyed Mehdi Rezayat Sorkhabadi, Aziz Mahmoudzadeh, Sepideh Arbabi Bidgoli, Masoumeh Heshmati, Samideh Khoei |
| المصدر: | The Nucleus. 62:221-225 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0303 health sciences, Chemistry, Chinese hamster ovary cell, 030302 biochemistry & molecular biology, Cell Biology, medicine.disease_cause, Molecular biology, In vitro, Silver nanoparticle, 03 medical and health sciences, chemistry.chemical_compound, In vivo, Bromide, Genetics, medicine, Molecular Medicine, Micronucleus, Cytotoxicity, Molecular Biology, Genotoxicity, 030304 developmental biology |
| الوصف: | Biomedical and pharmaceutical products comprising silver nanoparticles are attracting interest due to their potent antibacterial activities. For their safe use it has become imperative to test their cyto-genotoxic potential. In the present study the cytotoxicity and genotoxicity of three different sizes of AgNPs ranging from 15 to 22 nm and at concentrations 0.005–500 μg/ml were studied in Chinese Hamster ovary cell line (CHO-K1) cells. Cytoxicity was assessed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and for genotoxicity comet, and micronucleus assays were utilized. AgNPs were able to internalize CHO-K1 cells and cause cytotoxicity at concentrations 0.005–500 μg/ml. AgNP‐induced cyto-genotoxicity in CHO-K1 cells could be attributed to its smaller primary size. AgNP-C of size ~ 15 nm was the most potent among the three AgNPs. The genotoxic response was biphasic that increased at lower concentrations (0.005–0.025 μg/ml) and decreased at higher concentrations (0.05–0.1 μg/ml) after 24 h of exposure. Such potential in vitro genotoxic effect of AgNPs remains to be further confirmed in animal cells in vivo. |
| تدمد: | 0976-7975 0029-568X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::ac074ebce0808d75efd1e0ab07ca1bc3 https://doi.org/10.1007/s13237-019-00295-y |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........ac074ebce0808d75efd1e0ab07ca1bc3 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 09767975 0029568X |
|---|