Poly (Patton and Reeder’s) modified carbon paste electrode was fabricated and it was employed to study the electrochemical response of folic acid (FA) in phosphate buffer solution (PBS) of pH 7.4 by cyclic voltammetric (CV) technique. The results obtained at Poly (Patton and Reeder’s) modified carbon paste electrode shows a good electrocatalytic activity towards the oxidation of FA. The lower limit of detection of FA was found to be 1.88×10-8 M by CV technique. The parameters like effect of concentration and scan rate reveal the electrode process was controlled by adsorption of an analytes. The poly (Patton and Reeder’s) modified carbon paste electrode was used for the simultaneous determination of FA and uric acid (UA) at physiological pH.