Heparinoides naturais isolados de rodofíceas (Halymenia sp.) arribadas na costa cearense
| العنوان: | Heparinoides naturais isolados de rodofíceas (Halymenia sp.) arribadas na costa cearense |
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| المؤلفون: | Alexandre Holanda Sampaio, Daniel Barroso de Alencar, Wladimir Ronald Lobo Farias, Valeska Martins Torres, José Ariévilo Gurgel Rodrigues |
| المصدر: | Acta Scientiarum. Biological Sciences. 32 |
| بيانات النشر: | Universidade Estadual de Maringa, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Chromatography, biology, medicine.diagnostic_test, Extraction (chemistry), Red algae, Fractionation, biology.organism_classification, General Biochemistry, Genetics and Molecular Biology, Halymenia, Microbiology, chemistry.chemical_compound, Papain, chemistry, Agarose gel electrophoresis, medicine, General Agricultural and Biological Sciences, Sodium acetate, Partial thromboplastin time |
| الوصف: | The increasing demand for heparin (HEP) has led to a search for alternative sources of natural anticoagulants. This study aimed to evaluate the anticoagulant activity of sulfated polysaccharides (SP) isolated from a Halymenia rhodophyceae genus native to the coast of Ceara, Brazil. Total SP were obtained by consecutive digestions with papain in 0.1 M sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by ion-exchange chromatography on DEAE-cellulose column. The obtained fractions were concentrated by lyophilization and submitted to 0.5% agarose gel electrophoresis. Anticoagulant activity was evaluated by the activated partial thromboplastin time (APTT) using plasma from rabbits and a standard HEP (100 IU mg -1 ) curve. The extractions (53.96%) showed marked differences during the fractionation and in the degree of purification of SP. The species SP showed higher activity anticoagulant than that of HEP. However, the APTT of the fractions changed sharply among the extractions, expressing itself in a dose-dependent manner and increasing from 110.40 (1 st extraction) to 143.10 IU mg -1 (3 rd extraction). The results suggest that the anticoagulant activity of SP isolated from Halymenia sp. was promoted by inhibition of the intrinsic and/or common pathway of the coagulation cascade. The changes on APTT possibly will be elucidated through the mechanisms of action involved in coagulation and structural characterization of these compounds. Therefore, the red alga Halymenia sp. is a good source of heparinoids, and studies are suggested on the cultivation of this species and on the protection of natural algae banks. |
| تدمد: | 1807-863X 1679-9283 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::b179508242fb3fc1ebb0b9b25f00a47b https://doi.org/10.4025/actascibiolsci.v32i3.6282 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........b179508242fb3fc1ebb0b9b25f00a47b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1807863X 16799283 |
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