Effect of short-term light- and UV-stress on DMSP, DMS, and DMSP lyase activity in Emiliania huxleyi

التفاصيل البيبلوغرافية
العنوان: Effect of short-term light- and UV-stress on DMSP, DMS, and DMSP lyase activity in Emiliania huxleyi
المؤلفون: William G. Sunda, Michel Gosselin, Isabelle Laurion, Michel Lavoie, Michael Scarratt, Maurice Levasseur, Louise Darroch, Sonia Michaud, Gitane Caron
المصدر: Aquatic Microbial Ecology. 74:173-185
بيانات النشر: Inter-Research Science Center, 2015.
سنة النشر: 2015
مصطلحات موضوعية: Antioxidant, biology, CCMP, Coccolithophore, medicine.medical_treatment, fungi, Sulfur cycle, Aquatic Science, Dimethylsulfoniopropionate, biology.organism_classification, chemistry.chemical_compound, chemistry, Photosynthetically active radiation, Environmental chemistry, Botany, Phytoplankton, medicine, Ecology, Evolution, Behavior and Systematics, Emiliania huxleyi
الوصف: The ecological conditions and cellular mechanisms which affect the production of dimethylsulfoniopropionate (DMSP) and dimethylsulfide (DMS) in marine ecosystems are still enigmatic. This information is crucial for deriving accurate oceanic ecosystem models for the dynamics of these major players in the Earth’s sulfur cycle and climate. In the present study, we examined the effect of short-term increases in photosynthetically active radiation (PAR) and ultraviolet radiation (UVR) on the production of DMSP and DMS and on DMSP lyase potential activity (DLPA) in an axenic culture of the coccolithophore Emiliania huxleyi (CCMP 1742). Algal cells were subjected to a rapid shift from a low intensity of PAR (50 μE m-2 s-1; low light, LL) to a high intensity of PAR (198 μE m-2 s-1) and elevated UVR conditions (high light, HL), simulating what may occur during upward mixing in the surface mixed layer or during changes in cloud cover. During the 4.5 h exposure to HL, the intracellular DMSP normalized to cell volume increased by ca. 30%, and dissolved DMSP doubled relative to control values. However, the DLPA per unit of cell volume decreased by ~45% compared to the control value. The up-regulation of cellular DMSP concentration is consistent with an antioxidant and/or energy dissipation role for DMSP. The decrease in DLPA may indicate that the DMSP lyase enzyme plays no role in antioxidant protection in this algal species, but rather serves some other cellular function, such as grazing protection.
تدمد: 1616-1564
0948-3055
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::b2463db7fbaac8f88f3b1e182232ca9c
https://doi.org/10.3354/ame01735
رقم الأكسشن: edsair.doi...........b2463db7fbaac8f88f3b1e182232ca9c
قاعدة البيانات: OpenAIRE