Purpose: Determine the changes of saponin content in Paris polyphylla and analyze the correlation among them to provide theoretical basis for determining the best harvest scheme and clinical Paris polyphylla research; determine the similarity between wild Paris polyphylla and statutory Paris polyphylla, and explore expanding base sources of Paris polyphylla. Method: Adopt HPLC technology, build fingerprint of Paris polyphylla, and determine the content of all polyphyllin. Results: The contents of polyphyllin in different growing years have obvious difference, and the correlation among the compositions in the classification of dioscin is significant, while the correlation among the compositions in the classification of pennogenin is not obvious; the fingerprint of Paris delavayi Franchet and Paris mairei Leveille and the similarity of statutory Paris polyphylla are basically identical. Conclusion: The content of polyphyllin is not the single accumulation of time, it also closely connected with temperature, illumination, humidity and other environment in the growth cycle; the evaluation of the quality of Paris polyphylla should build standard colour spectra and fingerprint, and combine the changes of contents of main index components, thus, the quality of Paris polyphylla can be controlled effectively.
