Тема вÑпÑÑкной квалиÑикаÑионной ÑабоÑÑ: «ÐейÑÑвие микоÑокÑинов Stagonospora cirsii на ÑоÑÐ¾Ñ Ð¸Ð¼Ð¸ÑеÑкÑÑ Ð°ÐºÑивноÑÑÑ ÑоÑоÑиÑÑÐµÐ¼Ñ 2 пÑоÑоÑÑков ÑÑменÑ». ÐзмеÑÐµÐ½Ð¸Ñ Ð±ÑÑÑÑÑÑ OJIP-кинеÑик ÑлÑоÑеÑÑенÑии Ñ Ð»Ð¾ÑоÑилла а ÑвлÑеÑÑÑ Ð´Ð¾ÑÑовеÑнÑм меÑодом оÑенки влиÑÐ½Ð¸Ñ Ð³ÐµÑбиÑидов на ÑизиологиÑеÑкое ÑоÑÑоÑние ÑаÑÑений. ÐейÑÑвие двÑÑ Ð¼Ð¸ÐºÐ¾ÑокÑинов (ÑÑагонолид Ð, геÑбаÑÑмин I) и геÑбиÑида Ð´Ð¸ÐºÐ²Ð°Ñ Ð¸Ð·ÑÑалоÑÑ Ð½Ð° пÑоÑоÑÑÐºÐ°Ñ ÑÑÐ¼ÐµÐ½Ñ Hordeum vulgare L. ФлÑоÑеÑÑенÑÐ¸Ñ Ñ Ð»Ð¾ÑоÑилла а оÑенивали ÑеÑез 3, 24 и 48 ÑаÑов поÑле обÑабоÑки лиÑÑовÑÑ Ð´Ð¸Ñков и коÑней ÑелÑÑ Ð¿ÑоÑоÑÑков Ñ 30 мин Ñемновой адапÑаÑией пеÑед измеÑениÑми. ÐеÑбиÑид Ð´Ð¸ÐºÐ²Ð°Ñ Ð·Ð° 24 ÑаÑа пÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ð²Ñе клеÑки лиÑÑа к гибели, оказÑÐ²Ð°Ñ Ð±Ð¾Ð»ÐµÐµ моÑное дейÑÑвие на ФС2 по ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñо ÑÑагонолидом Ри геÑбаÑÑмином I. СÑагонолид РвÑзÑÐ²Ð°ÐµÑ Ð·Ð½Ð°ÑиÑелÑное изменение OJIP-кинеÑик ÑеÑез 48 ÑаÑов обÑабоÑки лиÑÑовÑÑ Ð´Ð¸Ñков, ÑÑо ÑказÑÐ²Ð°ÐµÑ Ð½Ð° ÑоÑможение пеÑеноÑа ÑлекÑÑонов в ФС2 до плаÑÑÐ¾Ñ Ð¸Ð½Ð¾Ð½Ð° Qa. СÑагонолид РпÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ñ ÐºÐ¾Ð»Ð¸ÑеÑÑва ÑоÑоÑиÑÑем в лиÑÑÑÑÑ ÑелÑÑ Ð¿ÑоÑоÑÑков в оÑлиÑие Ð¾Ñ Ð³ÐµÑбаÑÑмина I, коÑоÑÑй не оказÑÐ²Ð°ÐµÑ Ñакого ÑÑÑекÑа. ÐÑи обÑабоÑке лиÑÑовÑÑ Ð´Ð¸Ñков меÑодом инÑилÑÑÑаÑии двÑÐ¼Ñ Ð¸Ð·ÑÑеннÑми ÑиÑоÑокÑинами ÑолÑко геÑбаÑÑмин I вÑзÑÐ²Ð°ÐµÑ Ð²ÑÑаженнÑе Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ OJIP кинеÑик (ÑÑкоÑение OJ и JI Ñаз). Таким обÑазом, ÑезÑлÑÑаÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑабоÑÑ ÑказÑваÑÑ Ð½Ð° поÑенÑиалÑнÑÑ Ð¿ÐµÑÑпекÑивноÑÑÑ ÑазÑабоÑки веÑеÑÑв Ñ Ð³ÐµÑбиÑидной акÑивноÑÑÑÑ Ð½Ð° оÑнове микоÑокÑинов ÑÑагонолида Ри геÑбаÑÑмина I. The topic of the bachelor thesis: "The effect of mycotoxins of Stagonospora cirsii on the photochemical activity of photosystem 2 of barley seedlings". Measurements of fast OJIP kinetics of chlorophyll a fluorescence are a reliable method for assessing the effect of herbicides on the physiological state of plants. The effect of two mycotoxins (stagonolide A, herbarumin I) and the herbicide diquat was studied on seedlings of Hordeum vulgare L. Chlorophyll a fluorescence was evaluated 3, 24 and 48 hours after treatment of leaf discs and roots of whole seedlings with 30 min dark adaptation before measurements. herbicide diquat led to the death of all cells already after 24 h of exposure, being a more potent inhibitor of PS2 than stagonolide A or herbarumin I. Stagonolide A caused significant changes in OJIP-kinetics after 48 h of treatment of leaf discs, indicating an inhibition of electron transport within PS2 before Qa. Stagonolide A also lowered the numbers of PS2 complexes in leaves of whole seedlings, contrarily to herbarumin I that did not show this effect. When leaf discs were infiltrated with the abovementioned mycotoxins, then only herbarumin I was shown to significantly influence OJIP- kinetics in that both OJ and JI phases were accelerated. In summary, the obtained results confirmed the potential of both studied mycotoxins, stagonolide A and herbarumin I, to serve as the basic substances for the development of novel herbicides.
