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Rapid and efficient identification protocols forXanthomonas campestrispv.citrifor quarantine investigations

التفاصيل البيبلوغرافية
العنوان: Rapid and efficient identification protocols forXanthomonas campestrispv.citrifor quarantine investigations
المؤلفون: D.‐C. Park, John M. Young
المصدر: New Zealand Journal of Crop and Horticultural Science. 34:195-205
بيانات النشر: Informa UK Limited, 2006.
سنة النشر: 2006
مصطلحات موضوعية: Canker, Horticulture, Biology, medicine.disease, biology.organism_classification, DNA sequencing, Xanthomonas campestris, law.invention, Microbiology, law, Citrus canker, Quarantine, medicine, Primer (molecular biology), Agronomy and Crop Science, Pathogen, Bacteria
الوصف: The bacterial pathogen, Xanthomonas campestris pv. citri Group A, causing Oriental canker of Citrus spp., is regarded as a serious microbial threat to New Zealand horticulture. Rapid identification following a breach of biosecurity is essential if eradication is to be achieved. Confirmation of the presence of Oriental canker pathogen can be achieved with a single primer set obtained by DNA subtraction. Primers to X campestris pv. citri Group B, a pathogen of lesser severity, causing a cankrosis B or false canker, were also developed to discriminate these two pathogens. Specificity of primers for identification of these pathogens was investigated, and the sensitivity of primers for the detection of a specific pathogen in the field was assessed using X campestris pv. pruni as a surrogate pathogen. The pathogen can be detected from plant tissue without isolating the bacterium by amplifying DNA sequences specific to the pathogen from contaminated tissue washings.
تدمد: 1175-8783
0114-0671
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::b62d5aa49b5bf1d393337aa48fc58bbd
https://doi.org/10.1080/01140671.2006.9514408
حقوق: OPEN
رقم الأكسشن: edsair.doi...........b62d5aa49b5bf1d393337aa48fc58bbd
قاعدة البيانات: OpenAIRE
الوصف
تدمد:11758783
01140671