Magnetometric Evaluation for Cytotoxicity of Potassium Octatitanate Whisker on Alveolar Macrophages of Fischer 344 Rats
| العنوان: | Magnetometric Evaluation for Cytotoxicity of Potassium Octatitanate Whisker on Alveolar Macrophages of Fischer 344 Rats |
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| المؤلفون: | Yoshiharu Aizawa, Yuichiro Kudo, Mitsushi Okada, Toshihiko Satoh, Mitsuyasu Watanabe, Kaori Shibata, Masato Niitsuya, Makoto Kotani |
| المصدر: | Journal of Occupational Health. 44:321-328 |
| بيانات النشر: | Wiley, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Lung, medicine.diagnostic_test, Public Health, Environmental and Occupational Health, Molecular biology, In vitro, chemistry.chemical_compound, medicine.anatomical_structure, Bronchoalveolar lavage, chemistry, Apoptosis, Lactate dehydrogenase, medicine, Alveolar macrophage, Cytotoxic T cell, Cytotoxicity |
| الوصف: | Alveolar macrophages are thought to play a major role in the pathophysiology of lung diseases caused by exposure to various kinds of pathogens and particles. In the present study, the cytotoxic effect of potassium octatitanate whisker(PT) on macrophages was evaluated by means of magnetometry, lactate dehydrogenase (LDH) release measurement, apoptosis measurement and morphological observations. Alveolar macrophages obtained from Fischer rats (F344/N Slc) by bronchoalveolar lavage were incubated in vitro for 18 h with Fe3O4 as a magnetometric indicator, and PTs as test materials. In the control group and the group exposed to 10 μg/ml of PT, rapid attenuation of the remanent magnetic field (RMF), so called “relaxation, ” was observed immediately after cessation of the external magnetic field. In comparison, a delay of relaxation was observed in alveolar macrophages exposed to 20 and 40 μg/ml of PT. The decay constants, which are calculated from decreasing RMF for the first 2 min, in the groups exposed to 20 and 40 μg/ml of PT had significantly lower values than the control. LDH release induced by exposure to 20 and 40 μg/ml of PT increased significantly in a concentration dependent manner in PT-exposed macrophages, whereas only negligible LDH release was observed in control groups. The level of PT affecting alveolar macrophages was at the same concentration, and in a dose-dependent manner among relaxation, decay constant and LDH measurement. A DNA ladder detection method and morphological observations detected no apoptosis in PT-exposed macrophages. Electron microscopic examination revealed vacuolar changes and cell membrane damage in PT-exposed macrophages, but no significant changes in control macrophages. The results of magnetometry, LDH release, apoptosis measurement and electron microscopic observations suggest concentration dependent cytotoxicity caused by exposure of alveolar macrophages to PT. |
| تدمد: | 1348-9585 1341-9145 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::b898e212fb4170b98f9ae8808b1b8649 https://doi.org/10.1539/joh.44.321 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........b898e212fb4170b98f9ae8808b1b8649 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13489585 13419145 |
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