Nocardioides okcheonensis sp. nov., isolated from riverside soil
| العنوان: | Nocardioides okcheonensis sp. nov., isolated from riverside soil |
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| المؤلفون: | Ju Hee Kim, You Ju Ham, Seung Bum Kim |
| المصدر: | International Journal of Systematic and Evolutionary Microbiology. 73 |
| بيانات النشر: | Microbiology Society, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | General Medicine, Microbiology, Ecology, Evolution, Behavior and Systematics |
| الوصف: | An actinobacterial strain designated MMS20-HV4-12T, displaying a high hydrolytic potential for various substrates, was isolated from a riverside soil sample and characterized by polyphasic taxonomic analysis. Growth occurred at 10–37 °C (optimum, 30°C), with NaCl concentrations of 0–4 % (optimum, 0 %) and at pH 7–9 (optimum, pH 8). MMS20-HV4-12T was catalase-positive, oxidase-negative, rod-shaped and formed creamy white-coloured colonies. Based on the results of 16S rRNA gene sequence analysis, MMS20-HV4-12T was found to be mostly related to the type strains of Nocardioides alpinus (98.3 % sequence similarity), Nocardioides furvisabuli (98.1 %) and Nocardioides zeicaulis (98.0 %). MMS20-HV4-12T showed optimal growth on Reaoner's 2A agar, forming white-coloured colonies. The diagnostic polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol, the major fatty acids were iso-C16 : 0, C17 : 1 ω8c and 10-methyl-C17 : 0, the major isoprenoid quinone was MK-8(H4), the diagnostic cell-wall sugar was galactose, and the cell-wall diamino acid was ll-diaminopimelic acid. The genome of MMS20-HV4-12T was 4.47 Mbp in size with a G+C content of 72.9 mol%. The genome based analysis indicated low relatedness between MMS20-HV4-12T and all compared species of Nocardioides , as the highest digital DNA–DNA hybridization and the orthologous average nucleotide identity values were 26.8 and 83.8% respectively. Based on genotypic, phenotypic and phylogenomic characterization, MMS20-HV4-12T evidently represents a novel species of genus Nocardioides , for which the name Nocardioides okcheonensis sp. nov. (type strain=MMS20-HV4-12T=KCTC 49651T=LMG 32360T) is proposed. |
| تدمد: | 1466-5034 1466-5026 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::b942255441ba43a3d8bc91207d476e69 https://doi.org/10.1099/ijsem.0.005755 |
| رقم الأكسشن: | edsair.doi...........b942255441ba43a3d8bc91207d476e69 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14665034 14665026 |
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