Cytotoxicity and fluorescence studies of silica-coated CdSe quantum dots for bioimaging applications
| العنوان: | Cytotoxicity and fluorescence studies of silica-coated CdSe quantum dots for bioimaging applications |
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| المؤلفون: | Vijayamma Raji, C.S. Rejiya, Annie John, Annie Abraham, R. Vinayakan, Naresh S. Vinesh, M. Vibin |
| المصدر: | Journal of Nanoparticle Research. 13:2587-2596 |
| بيانات النشر: | Springer Science and Business Media LLC, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Neutral red, Materials science, technology, industry, and agriculture, Analytical chemistry, Bioengineering, General Chemistry, equipment and supplies, Condensed Matter Physics, Fluorescence, Atomic and Molecular Physics, and Optics, chemistry.chemical_compound, chemistry, Quantum dot, Modeling and Simulation, General Materials Science, Trypan blue, Viability assay, Luminescence, Cytotoxicity, Trioctylphosphine oxide, Nuclear chemistry |
| الوصف: | The toxicological effects of silica-coated CdSe quantum dots (QDs) were investigated systematically on human cervical cancer cell line. Trioctylphosphine oxide capped CdSe QDs were synthesized and rendered water soluble by overcoating with silica, using aminopropyl silane as silica precursor. The cytotoxicity studies were conducted by exposing cells to freshly synthesized QDs as a function of time (0–72 h) and concentration up to micromolar level by Lactate dehydrogenase assay, MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay, Neutral red cell viability assay, Trypan blue dye exclusion method and morphological examination of cells using phase contrast microscope. The in vitro analysis results showed that the silica-coated CdSe QDs were nontoxic even at higher loadings. Subsequently the in vivo fluorescence was also demonstrated by intravenous administration of the QDs in Swiss albino mice. The fluorescence images in the cryosections of tissues depicted strong luminescence property of silica-coated QDs under biological conditions. These results confirmed the role of these luminescent materials in biological labeling and imaging applications. |
| تدمد: | 1572-896X 1388-0764 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::bb5724d1ec395c75a237daf9d6b2bc51 https://doi.org/10.1007/s11051-010-0151-8 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........bb5724d1ec395c75a237daf9d6b2bc51 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 1572896X 13880764 |
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