Sorting of circulating tumor cells based on the microfluidic device of a biomimetic splenic interendothelial slit array
| العنوان: | Sorting of circulating tumor cells based on the microfluidic device of a biomimetic splenic interendothelial slit array |
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| المؤلفون: | Li Xiaobing, Wenhu Xu, Hongwei Tian, Jianfeng Chen, Meirong Yi, Zefan Yang, Zhong Min |
| المصدر: | Microfluidics and Nanofluidics. 25 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Materials science, 010401 analytical chemistry, Microfluidics, 02 engineering and technology, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 01 natural sciences, Slit, 0104 chemical sciences, Electronic, Optical and Magnetic Materials, law.invention, Cell membrane, chemistry.chemical_compound, medicine.anatomical_structure, Circulating tumor cell, Flow velocity, Silicon nitride, chemistry, law, Materials Chemistry, medicine, Fluidics, 0210 nano-technology, Filtration, Biomedical engineering |
| الوصف: | In this paper, we focused on a microfluidic sorting method based on bionic splenic sinus microstructures to capture circulating tumor cells (CTCs). A dynamic multiphase fluidic model was developed to explore the effects of different flows and the parameters of the spleen-specific structure of the interendothelial slit (IES) on the cell membrane strain, as represented by the interface area between two phases. The results indicated that parameters of the IES and flow velocity strongly influence cell membrane strain. The biomimetic IES structure has more advantages than do circular pores because the slit structure has a lower flow resistance compared to the circle structure. A microfluidic device based on a biomimetic IES with ultrathin (500 nm thick) silicon nitride filters was designed and fabricated for high-throughput enrichment of high-viability CTCs. The silicon nitride filters had areas as large as 36 mm2 (6 × 6 mm) and included nearly 18,000 slit units, which was conducive to obtaining a high-throughput device. Moreover, the effects of different parameters, such as velocity, slit width, dilution ratio and solution volume, on the cell capture efficiency and cell viability were explored. The results show that the microfluidic device based on a biomimetic IES has a high potential for preserving viable cells. This study quantitatively explored the effects of different parameters on cell viability during the CTC physical filtration process. Additionally, this study will be helpful for designing high-throughput CTC enrichment devices that capture high-viability cells. |
| تدمد: | 1613-4990 1613-4982 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::bf4f2dc6b24168b64d58ddef2e49652a https://doi.org/10.1007/s10404-021-02459-2 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........bf4f2dc6b24168b64d58ddef2e49652a |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 16134990 16134982 |
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