Background: This study aimed to investigate the rational medication use of health students, their health literacy (HL), the associations of these factors with sociodemographic factors, and the contribution of HL to the rational use of medication (RUM). Methods: Data for this cross-sectional study were collected from 1256 health students between February and June 2020 in Turkey, using a Sociodemographic Information Form, the Rational Medication Use Scale, and the Turkish version of the European HL Scale. Results: The sociodemographic characteristics of the participants were not associated with their RUM. HL level was higher in the participants who were using medication regularly, those over the age of 20, and those with good income levels (P