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Comparison of three human liver cell lines for in vitro drug-induced liver injury assessment: Huh7, HepaRG, and stem cell-derived hepatocytes

التفاصيل البيبلوغرافية
العنوان: Comparison of three human liver cell lines for in vitro drug-induced liver injury assessment: Huh7, HepaRG, and stem cell-derived hepatocytes
المؤلفون: Hae Chan Ha, Dae Kyong Kim, Ju Yeun Kim, Moon Jung Back, Ji Min Jang, So Yoon Yun, Hee Soo Kim
المصدر: Molecular & Cellular Toxicology. 15:271-285
بيانات النشر: Springer Science and Business Media LLC, 2019.
سنة النشر: 2019
مصطلحات موضوعية: 0301 basic medicine, Drug, Liver injury, business.industry, Health, Toxicology and Mutagenesis, media_common.quotation_subject, Public Health, Environmental and Occupational Health, Pharmacology, Toxicology, medicine.disease, In vitro, Pathology and Forensic Medicine, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Cell culture, 030220 oncology & carcinogenesis, medicine, Secretion, General Pharmacology, Toxicology and Pharmaceutics, Stem cell, business, Cytotoxicity, Drug metabolism, media_common
الوصف: Backgrounds: Drug-induced liver injury (DILI) is a major causal factor for failure in clinical trials and withdrawal of drugs from pharmaceutical markets. Therefore, a human cell-based in vitro system has been established to overcome the limitations of preclinical trials. However, previous studies focused on the drug metabolism in each cell line or described to be superior in one single cell line for assessing DILI. In this study, we used Huh7, HepaRG, and Hepatosight-S cells to evaluate the liver toxicity and dysfunction driven by DILI-inducing drugs. Methods: Cytotoxicity of 17 DILI-inducing drugs was assessed by LDH release assay and the drug-induced liver dysfunction was confirmed by albumin secretion. Furthermore, we analyzed the expression levels of phase I and phase II enzymes and nuclear receptors in each cell lines. Results: DILI-inducing drugs were differently detected in each cell line because each cell line differs on the expression of drug metabolic enzymes which associated with reactivity on DILI inducing drugs. Conclusion: To predict the responses of DILI-inducing drugs in human liver cells, using diverse cell lines having different drug metabolic capabilities is more suitable for predicting DILI.
تدمد: 2092-8467
1738-642X
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::c1f914e85df6c8ff03b6720308bd3666
https://doi.org/10.1007/s13273-019-0031-y
حقوق: CLOSED
رقم الأكسشن: edsair.doi...........c1f914e85df6c8ff03b6720308bd3666
قاعدة البيانات: OpenAIRE
الوصف
تدمد:20928467
1738642X