The Use of Thermal Dissociation for Selection of DNA Aptamers
| العنوان: | The Use of Thermal Dissociation for Selection of DNA Aptamers |
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| المؤلفون: | George S. Krasnov, O. S. Volkova, S. P. Radko, V. E. Shershov, Alexander S. Zasedatelev, Sergey A. Lapa, V. E. Kuznetsova, Alexander V. Chudinov |
| المصدر: | Russian Journal of Bioorganic Chemistry. 46:551-556 |
| بيانات النشر: | Pleiades Publishing Ltd, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, 010405 organic chemistry, Oligonucleotide, Aptamer, Organic Chemistry, DNA Aptamers, 01 natural sciences, Biochemistry, Combinatorial chemistry, 0104 chemical sciences, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, chemistry, Thermal dissociation, Bioorganic chemistry, DNA, Selection (genetic algorithm), Conjugate |
| الوصف: | A method of thermal dissociation of DNA–protein conjugates was developed for the selection of aptamers to human alpha-fetoprotein. The method is based on the identification of the DNA oligonucleotides from the combinatorial DNA libraries with the highest specificity to targets, which remained within the conjugate after two stages: a) washing of unbound sequences; and b) removal of the sequences bound to the DNA but displaying a low affinity to it. The undestroyed most stable complexes were subjected to the thermal dissociation with the involvement of the corresponding oligonucleotides in the next rounds of selection. A comparison of the proposed method with the standard washing demonstrated a more intensive enrichment of the library. Due to the tighter conditions for washing of the target-bound DNA oligonucleotides the use of thermal dissociation can increase the efficiency of selection of highly specific aptamers to their molecular targets. |
| تدمد: | 1608-330X 1068-1620 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::c3feddd3f0bac53b82a03b8ac357a6aa https://doi.org/10.1134/s106816202004010x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........c3feddd3f0bac53b82a03b8ac357a6aa |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 1608330X 10681620 |
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