TCR usage, gene expression and function of two distinct FOXP3 + Treg subsets within CD4 + CD25 hi T cells identified by expression of CD39 and CD45RO
| العنوان: | TCR usage, gene expression and function of two distinct FOXP3 + Treg subsets within CD4 + CD25 hi T cells identified by expression of CD39 and CD45RO |
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| المؤلفون: | Dmitriy M. Chudakov, Lei Jiang, Ekaterina V. Putintseva, Hill Gaston, Ting Li, Libin Zhang, Lingying Ye, Huji Xu, Giles S.H. Yeo, Li Lin, Jian Yin, Mikhail Shugay, Brian Y.H. Lam, Wei Liu, Xin Wu, Jane C. Goodall |
| المصدر: | Immunology & Cell Biology. 94:293-305 |
| بيانات النشر: | Wiley, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | 0301 basic medicine, Effector, T cell, Immunology, T-cell receptor, FOXP3, hemic and immune systems, chemical and pharmacologic phenomena, Cell Biology, Biology, Gene expression profiling, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Immune system, medicine.anatomical_structure, Antigen, medicine, Immunology and Allergy, IL-2 receptor, 030215 immunology |
| الوصف: | FOXP3+ regulatory T (Treg) cells are indispensable for immune homeostasis, but their study in humans is complicated by heterogeneity within Treg, the difficulty in purifying Tregs using surface marker expression (e.g. CD25) and the transient expression of FOXP3 by activated effector cells. Here, we report that expression of CD39 and CD45RO distinguishes three sub-populations within human CD4(+)CD25(hi) T cells. Initial phenotypic and functional analysis demonstrated that CD4(+)CD25(hi)CD39(+)CD45RO(+) cells had properties consistent with effector Treg, CD4(+)CD25(hi)CD39(-)CD45RO(-) cells were naive Treg and CD4(+)CD25(hi)CD39(-)CD45RO(+) cells were predominantly non-Treg with effector T-cell function. Differences in these two newly identified Treg subsets were corroborated by studies of gene expression and TCR analysis. To apply this approach, we studied these two newly identified Treg subsets in ankylosing spondylitis, and showed impairment in both effector and naive Treg. This work highlights the importance of discriminating Treg subsets to enable proper comparisons of immune regulatory capacity in healthy individuals and those with inflammatory disease. |
| تدمد: | 1440-1711 0818-9641 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::c4413e5d95da12d3b5af88043a682862 https://doi.org/10.1038/icb.2015.90 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........c4413e5d95da12d3b5af88043a682862 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14401711 08189641 |
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