Anti-Neuroinflammatory Effects of Houttuynia cordata Extract on LPS-Stimulated BV-2 Microglia
| العنوان: | Anti-Neuroinflammatory Effects of Houttuynia cordata Extract on LPS-Stimulated BV-2 Microglia |
|---|---|
| المؤلفون: | TK Park, Hyun Kang, S Koppula, SH Jung, Kim |
| المصدر: | Tropical Journal of Pharmaceutical Research. 12 |
| بيانات النشر: | African Journals Online (AJOL), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Lipopolysaccharide, biology, DPPH, Pharmaceutical Science, Interleukin, Pharmacology, biology.organism_classification, Nitric oxide, Houttuynia cordata, Nitric oxide synthase, chemistry.chemical_compound, chemistry, Biochemistry, biology.protein, Pharmacology (medical), Viability assay, Neuroinflammation |
| الوصف: | Purpose : To evaluate the anti-neuroinflammatory effects of Houttuynia cordata extract ( H. cordata ) in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells, and its anti-oxidant properties. Methods : Anti-oxidant properties were evaluated by 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. Cell viability was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyl-tetrazolium bromide (MTT) assay. LPS was used to stimulate BV-2 cells. Nitric oxide (NO) levels were measured using Griess assay. Inducible NO synthase (iNOS) expression, interleukin (IL)-6 expressional level were determined by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Results : Ethyl actetae (HC-EA) extract of H. cordata significantly scavenged DPPH free radicals in a concentration-dependent fashion. The increased levels of NO, iNOS and IL-6 in LPS-stimulated BV-2 microglial cells were also suppressed by HC-EA extract in a concentration-dependent manner. Conclusion: The result indicate that the HC-EA extract exhibited strong anti-oxidant properties and inhibited the excessive production of pro-inflammatory mediators, including NO, iNOS and IL-6, in LPSstimulated BV-2 cells. The anti-oxidant phenolic compounds present in HC-EA extract might play an important role in ameliorating neuroinflammatory processes in LPS-stimulated BV-2 microglial cells. Keywords: Houttuynia cordata , DPPH radicals, antioxidant, neuroinflammation, BV-2 cells, iNOS, COX-2, IL-6. |
| تدمد: | 1596-9827 1596-5996 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::c46f55b1d1227518e83d301df96c9c4a https://doi.org/10.4314/tjpr.v12i4.12 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........c46f55b1d1227518e83d301df96c9c4a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15969827 15965996 |
|---|