The invasive pest tomato pinworm, T. absoluta (Meyrick) (Lepidoptera: Gelechiidae) was observed first time in the Southern Rajasthan during 2018-2020 under field and protected cultivation in tomato. Morphological and molecular identification (amplifying cytochrome oxidase gene (CO-I) of mitochondrial DNA) was done to confirm the occurrence of this new invasive species. The development period of T. absoluta had four stages viz; eggs, larva, pupa and adult. The duration of the incubation period, first, second, third and fourth instar larvae were observed at 4.52±0.68, 2.38±0.50, 3.95±0.80, 3.43±0.51 and 2.57±0.51 days, respectively. The eggs hatching was observed at 94-97 per cent. The average length of first, second, third and fourth instar larvae was measured at 0.610±0.037, 1.577±0.067, 3.917±0.121 and 7.379±0.176 mm, respectively. The total larval period was observed as 10-15 days with a mean of 12.33±1.35 days. The pupal period was observed from 10-12 days with a mean of 10.95±0.86 days. The adult longevity on an average was 12.00±1.41 days with a range of 10-14 days. The complete life span of T. absoluta ranged from 30-40 days with a mean of 35.43±3.56 days.
