An SGS3-like protein functions in RNA-directed DNA methylation and transcriptional gene silencing in Arabidopsis

التفاصيل البيبلوغرافية
العنوان: An SGS3-like protein functions in RNA-directed DNA methylation and transcriptional gene silencing in Arabidopsis
المؤلفون: Sudesh Kumar Yadav, Zhimin Zheng, Wenbo Li, Yuanlei Hu, Jian-Kang Zhu, Xin-Jian He, Yu Xing, JeeEun Oh
المصدر: The Plant Journal. 62:92-99
بيانات النشر: Wiley, 2010.
سنة النشر: 2010
مصطلحات موضوعية: Genetics, Promoter, Cell Biology, Plant Science, Biology, RNA interference, DNA methylation, biology.protein, Demethylase, Gene silencing, Epigenetics, RNA-Directed DNA Methylation, Gene
الوصف: RNA-directed DNA methylation (RdDM) is an important epigenetic mechanism for silencing transgenes and endogenous repetitive sequences such as transposons. The RD29A promoter-driven LUCIFERASE transgene and its corresponding endogenous RD29A gene are hypermethylated and silenced in the Arabidopsis DNA demethylase mutant ros1. By screening for second-site suppressors of ros1, we identified the RDM12 locus. The rdm12 mutation releases the silencing of the RD29A-LUC transgene and the endogenous RD29A gene by reducing the promoter DNA methylation. The rdm12 mutation also reduces DNA methylation at endogenous RdDM target loci, including transposons and other repetitive sequences. In addition, the rdm12 mutation affects the levels of small interfering RNAs (siRNAs) from some of the RdDM target loci. RDM12 encodes a protein with XS and coiled-coil domains, and is similar to SGS3, which is a partner protein of RDR6 and can bind to double-stranded RNAs with a 5' overhang, and is required for several post-transcriptional gene silencing pathways. Our results show that RDM12 is a component of the RdDM pathway, and suggest that RdDM may involve double-stranded RNAs with a 5' overhang and the partnering between RDM12 and RDR2.
تدمد: 0960-7412
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::c7f71578c000a9f00f981f0878d9916f
https://doi.org/10.1111/j.1365-313x.2010.04130.x
حقوق: OPEN
رقم الأكسشن: edsair.doi...........c7f71578c000a9f00f981f0878d9916f
قاعدة البيانات: OpenAIRE