Spectral imaging of single molecules
| العنوان: | Spectral imaging of single molecules |
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| المؤلفون: | Alois Renn, Hermann Bach, Urs P. Wild |
| المصدر: | Single Molecules. 1:73-77 |
| بيانات النشر: | Wiley, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | medicine.medical_specialty, Spectrometer, business.industry, Chemistry, Clinical Biochemistry, General Chemistry, Chromophore, Slit, Fluorescence spectra, Molecular physics, General Biochemistry, Genetics and Molecular Biology, Spectral imaging, Optics, Fluorescence microscope, medicine, Molecule, Slit width, business, Molecular Biology |
| الوصف: | Using a setup based on fluorescence microscopy we study fluorescence spectra of single chromophores. Being point like emission sources single molecules can be focused to the entrance slit of a spectrometer. Even with narrow slit width the light passes the system with almost no loss. We show that, making full use of the emission properties of a single molecule, fluorescence spectra of many single molecules can be recorded simultaneously using (hyper-)spectral imaging. The vibrational frequencies of the single terrylene chromophores in naphthalene are the same within the accuracy of our experiments but the vibronic lines show pronounced differences in their relative intensities. |
| تدمد: | 1438-5171 1438-5163 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::c81bd0f3a103bda21086c492b5e618fc https://doi.org/10.1002/(sici)1438-5171(200004)1:1<73::aid-simo73>3.0.co;2-q |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........c81bd0f3a103bda21086c492b5e618fc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14385171 14385163 |
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