Sub-lethal Camphor Exposure Triggers Oxidative Stress, Cardiotoxicity, and Cardiac Physiology Alterations in Zebrafish Embryos
| العنوان: | Sub-lethal Camphor Exposure Triggers Oxidative Stress, Cardiotoxicity, and Cardiac Physiology Alterations in Zebrafish Embryos |
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| المؤلفون: | Ming-Zhe Zhang, Erwei Hao, Gilbert Audira, Nemi Malhotra, Zhong-Shang Xia, Jiagang Deng, Du Zhengcai, Ferry Saputra, Marri Jmelou M. Roldan, Hou Xiaotao, Yan-ting Wei, Chung-Der Hsiao |
| المصدر: | Cardiovascular Toxicology. 21:901-913 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Reactive oxygen species, biology, Developmental toxicity, Cinnamomum camphora, Pharmacology, Toxicology, Malondialdehyde, biology.organism_classification, medicine.disease_cause, Superoxide dismutase, chemistry.chemical_compound, Camphor, chemistry, medicine, biology.protein, Cardiology and Cardiovascular Medicine, Molecular Biology, Zebrafish, Oxidative stress |
| الوصف: | Camphor is a terpene ketone with aromatic and volatile properties in nature derived from the bark of Cinnamomum camphora or synthesized from turpentine. Camphor exhibits various biological properties such as anti-microbial, anti-viral, anti-coccidial, and anti-cancer. It is also used as a form of topical medication for skin irritation, joint pain, and as a relief for itching from insect bites. However, even though the high dose of camphor has been documented to be toxic/lethal in humans in different studies, camphor's developmental toxicity has not yet been explored, and its extensive mechanism of action is still unclear. In the present study, we aimed to assess the toxic effects of camphor in zebrafish embryos in the initial developmental stages. The obtained results demonstrated that a sub-lethal dose of camphor caused a decrease in hatching rate, body length, and substantial elevation in malformation rate on zebrafish embryos. On further observation, in the following time frame, curved body and pericardial edema of zebrafish were also observed. Furthermore, exposure to a sub-lethal dose of camphor was also able to trigger cardiotoxicity in zebrafish larvae. Later, on subsequent biochemical analysis, it was found that the antioxidant capacity inhibition and oxidative stress elevation that occurred after camphor exposure might be associated with the inhibition of total superoxide dismutase (SOD) activity and an increase in reactive oxygen species (ROS) and malondialdehyde (MDA) concentration. In addition, compared to the control group, several apoptotic cells in treated zebrafish were also found to be elevated. Finally, after further investigation on marker gene expressions, we conclude that the developmental toxicity of camphor exposure might be associated with apoptosis elevation and oxidative stress. Taken together, the current study provides a better understanding of the developmental toxicity of camphor on zebrafish, a promising alternative animal model to assess the developmental toxicity of chemical compounds. |
| تدمد: | 1559-0259 1530-7905 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::c8f03a665b3ca462453a50e72478a7af https://doi.org/10.1007/s12012-021-09682-x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........c8f03a665b3ca462453a50e72478a7af |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15590259 15307905 |
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