A novel process for obtaining phenylpropanoic acid precursor using Escherichia coli with a constitutive expression system
| العنوان: | A novel process for obtaining phenylpropanoic acid precursor using Escherichia coli with a constitutive expression system |
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| المؤلفون: | Jing-long Liang, Ping Sun, Bing-Hua Jiang, Liqiong Guo, Weixiong Guo, Hua Wan, Junfang Lin |
| المصدر: | Food Science and Biotechnology. 25:795-801 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, biology, Phenylalanine, Rhodotorula, BioBrick, biology.organism_classification, medicine.disease_cause, Phenylpropanoic acid, Applied Microbiology and Biotechnology, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, chemistry, Scientific method, Yield (chemistry), medicine, Organic chemistry, Tyrosine ammonia-lyase, Escherichia coli, Food Science, Biotechnology |
| الوصف: | Phenylpropanoids are widely used in food supplements, pharmaceuticals, and cosmetics with diverse benefits to human health. Trans-cinnamic acid or p-coumaric acid is usually used as the starting precursor to produce phenylpropanoids. Synthetic bioengineering of microbial cell factories offers a sustainable and flexible alternative method for obtaining these compounds. In this study, a constitutive expression system consisting of Rhodotorula glutinis phenylalanine/tyrosine ammonia lyase was developed to produce a phenylpropanoic acid precursor in Escherichia coli. To improve trans-cinnamic acid and p-coumaric acid production, BioBrick optimization was investigated, causing a 7.2- and 14.2-fold increase in the yield of these compounds, respectively. The optimum strain was capable of de novo producing 78.81 mg/L of trans-cinnamic acid and 34.67 mg/L of p-coumaric acid in a shake flask culture. The work presented here paves the way for the development of a sustainable and economical process for microbial production of a phenylpropanoic acid precursor. |
| تدمد: | 2092-6456 1226-7708 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::c958191e32b90853e48998f8374cdb4c https://doi.org/10.1007/s10068-016-0134-3 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........c958191e32b90853e48998f8374cdb4c |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 20926456 12267708 |
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