Liposome Mediated Transfer of Marker and Cytokine Genes Into Rat and Human Glioblastoma Cells in Vitro and in Vivo
| العنوان: | Liposome Mediated Transfer of Marker and Cytokine Genes Into Rat and Human Glioblastoma Cells in Vitro and in Vivo |
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| المؤلفون: | Wolfgang Walther, Stephan Dyballa, Ruth Greferath, Friedrich Weber, Jian-Hong Zhu, Regina Reszka |
| المصدر: | Journal of Liposome Research. 5:149-167 |
| بيانات النشر: | Informa UK Limited, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | In vivo, Cell culture, Pharmaceutical Science, lac operon, Cationic liposome, Transfection, Transfer technique, Biology, Marker gene, Molecular biology, In vitro |
| الوصف: | Gene transfer of Lac Z and TNF-α gene was performed in several glioblastoma cell lines in vitro (A172, F98, U373-MG, HS683, U343-MG, N31, N64, N28, N39 and N65). In comparison to the calcium phosphate precipitation technique (CPPT) the cationic liposomes were more successful measured by marker gene transfer (0.76 or 2.0%, depending on the cell line). In contrast, the amount of released human TNF-α was independed from the chosen transfer technique. Since the vector pWG29del3-hTNF contains a hormone response element it was possible to stimulate the hTNF secretion by dexamethasone up to 18 fold from a basic level of 1.1 ng/ml/24h/5×105 cells. A liposomal mediated marker gene transfer (Lac Z) into a rat glio-blastoma in vivo revealed a three layer area of transfected cells around the stereotactical injection channel. |
| تدمد: | 1532-2394 0898-2104 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::c9af94502a9c5dffba25a0edc51f41bd https://doi.org/10.3109/08982109509039915 |
| رقم الأكسشن: | edsair.doi...........c9af94502a9c5dffba25a0edc51f41bd |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 15322394 08982104 |
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