17 Effects of Indoor Air Pollution on Both a Healthy and 'an Inflamed' Model of the Alveolar Barrier in Vitro
العنوان: | 17 Effects of Indoor Air Pollution on Both a Healthy and 'an Inflamed' Model of the Alveolar Barrier in Vitro |
---|---|
المؤلفون: | Kirsty Meldrum, Stephen J Evans, Michael J Burgum, Shareen H Doak, Martin J D Clift |
المصدر: | Annals of Work Exposures and Health. 67:i44-i45 |
بيانات النشر: | Oxford University Press (OUP), 2023. |
سنة النشر: | 2023 |
مصطلحات موضوعية: | Public Health, Environmental and Occupational Health |
الوصف: | Exposure to indoor air pollutants (IAP) has increased recently, with people spending more time indoors. Increased exposures of IAP on a healthy population are poorly understood, and those with allergic respiratory conditions less so. The objective of this study, therefore, was to implement a well-characterised in vitro model of the human alveolar epithelial barrier (A549+PMA differentiated THP-1 incubated with/without IL-13, IL-5 and IL-4) to determine the effects of a standardised indoor particulate (NIST2583) on both a healthy lung model and modelling a type-II inflammatory response. Using concentrations from the literature, and an environmentally appropriate exposure we investigated 232, 464 and 608ng/cm2 of NIST2583 respectively. Membrane integrity (blue dextran), viability (trypan blue), genotoxicity (micronucleus (Mn) assay) and (pro-) and (anti-)inflammatory effects (IL-6, IL-8, IL-33, and IL-10) were then assessed 24hours post exposure to both models. No changes in Mn frequency or membrane integrity in either model was noted when exposed to any concentration of NIST2583 tested. Though, a significant decrease (p |
تدمد: | 2398-7316 2398-7308 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::cf78f97087b1dd590a5cfd7e2ea6d788 https://doi.org/10.1093/annweh/wxac087.108 |
حقوق: | CLOSED |
رقم الأكسشن: | edsair.doi...........cf78f97087b1dd590a5cfd7e2ea6d788 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 23987316 23987308 |
---|