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17 Effects of Indoor Air Pollution on Both a Healthy and 'an Inflamed' Model of the Alveolar Barrier in Vitro

التفاصيل البيبلوغرافية
العنوان:	17 Effects of Indoor Air Pollution on Both a Healthy and 'an Inflamed' Model of the Alveolar Barrier in Vitro
المؤلفون:	Kirsty Meldrum, Stephen J Evans, Michael J Burgum, Shareen H Doak, Martin J D Clift
المصدر:	Annals of Work Exposures and Health. 67:i44-i45
بيانات النشر:	Oxford University Press (OUP), 2023.
سنة النشر:	2023
مصطلحات موضوعية:	Public Health, Environmental and Occupational Health
الوصف:	Exposure to indoor air pollutants (IAP) has increased recently, with people spending more time indoors. Increased exposures of IAP on a healthy population are poorly understood, and those with allergic respiratory conditions less so. The objective of this study, therefore, was to implement a well-characterised in vitro model of the human alveolar epithelial barrier (A549+PMA differentiated THP-1 incubated with/without IL-13, IL-5 and IL-4) to determine the effects of a standardised indoor particulate (NIST2583) on both a healthy lung model and modelling a type-II inflammatory response. Using concentrations from the literature, and an environmentally appropriate exposure we investigated 232, 464 and 608ng/cm2 of NIST2583 respectively. Membrane integrity (blue dextran), viability (trypan blue), genotoxicity (micronucleus (Mn) assay) and (pro-) and (anti-)inflammatory effects (IL-6, IL-8, IL-33, and IL-10) were then assessed 24hours post exposure to both models. No changes in Mn frequency or membrane integrity in either model was noted when exposed to any concentration of NIST2583 tested. Though, a significant decrease (p
تدمد:	2398-7316 2398-7308
URL الوصول:	https://explore.openaire.eu/search/publication?articleId=doi_________::cf78f97087b1dd590a5cfd7e2ea6d788 https://doi.org/10.1093/annweh/wxac087.108
حقوق:	CLOSED
رقم الأكسشن:	edsair.doi...........cf78f97087b1dd590a5cfd7e2ea6d788
قاعدة البيانات:	OpenAIRE

الوصف
تدمد:	23987316 23987308