التفاصيل البيبلوغرافية
العنوان:
Modification of the larval swimming behavior inOikopleura dioica, a chordate with a miniaturized central nervous system by dsRNA injection into fertilized eggs
المؤلفون:
Lisbeth C. Olsen , Yana Mikhaleva , Daniel Chourrout , Orsolya Kreneisz , Joel C. Glover
المصدر:
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution . 324:114-127
بيانات النشر:
Wiley, 2015.
سنة النشر:
2015
مصطلحات موضوعية:
Gene knockdown , biology , Glutamate decarboxylase , Chordate , Anatomy , biology.organism_classification , Choline acetyltransferase , Cell biology , Oikopleura , Vesicular acetylcholine transporter , Genetics , medicine , Molecular Medicine , Animal Science and Zoology , Oikopleura dioica , Ecology, Evolution, Behavior and Systematics , Acetylcholine , Developmental Biology , medicine.drug
الوصف:
Using RNA interference, we have selectively perturbed neurotransmitter-related features of the larval swimming behavior of Oikopleura dioica, a tunicate with a central nervous system comprising about 130 neurons. We injected dsRNA into fertilized eggs to knockdown the expression of the genes, respectively, encoding ChAT (choline acetyltransferase) and GAD (glutamic acid decarboxylase), enzymes critical for the biosynthesis of acetylcholine and GABA. These two neurotransmitters have conserved roles during evolution, particularly within chordate motor systems, where they mediate respectively neuromuscular and central inhibitory signals. In Oikopleura, interference with ChAT expression prevented the normal bidirectional, propagating tail movement characteristic of swimming, permitting only repeated unilateral tail bends. Proper swimming was never observed, and the resting period between episodes of activity was lengthened. This phenotype is most likely caused by the reduction of transcription observed for both the targeted ChAT gene and the VAChT gene (Vesicular Acetylcholine Transporter), both genes being transcribed from the same operon. Interference with GAD expression led to an uncoordinated version of swimming with a spiral movement trajectory, but with episodes similar in duration and cycle frequency to those of normal swimming. Our results suggest locomotor functions for ChAT and GABA that are more subtle than previously proposed for tunicates and opens the way for a genetic dissection of Oikopleura neuronal circuits, which are likely to be among the most simplified in the chordate phylum. J. Exp. Zool. (Mol. Dev. Evol.) 324B: 114–127, 2015. © 2015 Wiley Periodicals, Inc.
تدمد:
1552-5007
URL الوصول:
https://explore.openaire.eu/search/publication?articleId=doi_________::cfb78904981e704e6349b8e56064a283 https://doi.org/10.1002/jez.b.22607
حقوق:
OPEN
رقم الأكسشن:
edsair.doi...........cfb78904981e704e6349b8e56064a283
قاعدة البيانات:
OpenAIRE
