DETERMINATION OF THE SPECIES DIVERSITY OF THE INTESTINAL MICROBIOME OF PIGS IN ORDER TO CREATE A CONSORTIUM OF MICROORGANISMS FOR WASTEWATER TREATMENT FROM ORGANIC POLLUTANTS
التفاصيل البيبلوغرافية
العنوان:
DETERMINATION OF THE SPECIES DIVERSITY OF THE INTESTINAL MICROBIOME OF PIGS IN ORDER TO CREATE A CONSORTIUM OF MICROORGANISMS FOR WASTEWATER TREATMENT FROM ORGANIC POLLUTANTS
The species diversity of the porcine intestinal microbiome wasdetermined. This study was performed using sequencing on the Ion TorrentPGM platform. The intestines of the pig are represented by phyla: Firmicutes– 53.1%, Bacteroidota – 21.9%, Proteobacteria – 15.6%, Actinobacteriota– 6.3%, Verrucomicrobiota – 3.1%; 7 classes of microorganisms have been identified. Microorganisms of the class Bacteroidia and Clostridia predominate.An analysis of the microbiome at the order level showed that of the 12 ordersof bacteria found in the gastrointestinal tract (GIT) of the pig, representativesof Oscillospirales, Bacteroidales and Lachnospirales predominate. The genericdiversity of the pig microbiome was represented by 32 bacteria. Of the familiesPrevotella and Ruminococcaceae prevailing in the GIT of the pig, bacteria ofthe genera Faecalibacterium and Prevotella were the most abundant. Basedon the experimental data obtained, representatives of the genera Escherichia,Proteus, Staphylococcus, Azotobacter were selected to create a consortium ofmicroorganisms used in wastewater treatment from pollution.
