V cells: A novel antigen specific immunoglobulin expressing cell lineage in immunized animals distinct from classical B cell populations (P4386)
| العنوان: | V cells: A novel antigen specific immunoglobulin expressing cell lineage in immunized animals distinct from classical B cell populations (P4386) |
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| المؤلفون: | Efthalia Chronopoulou, Alejandro Uribe-Benninghoff, Jeanne Elia, Andrea Nguyen, Jacob Rabenstein, Janean Fisher, Jody Berry |
| المصدر: | The Journal of Immunology. 190:52.13-52.13 |
| بيانات النشر: | The American Association of Immunologists, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | Following immunization with protein antigens, an uncharacterized immunoglobulin bearing antigen specific cell population was identified and named as "V cells". This cell population lacks surface expression of B cell lineage specific markers such as B220, CD19, as well as T cell, NK, NKT, basophil and stem cell specific markers as determined by flow cytometric analysis. V cells are characterized as being positive for CD24, CD43, CD45, CD49b, CD48, CD79b and IgG. They are observed in the spleen, bone marrow, and blood of immunized mice but were not detected in other tissues such as the thymus, lymph nodes and peritoneal exudate cells. Antigen specific V cells were generated following immunization of at least 6 different protein antigens and appeared as early as 7 to 14 days following a single immunization. Molecular analysis of isolated mRNA from bulk sorted V cells proved that this novel cell population produces antigen specific Ig. Molecular analysis was also performed on the cDNA of individually and bulk sorted V cells extracted from the bone marrow and spleen. Immunoglobulin VH genes were then sequenced from each cDNA pool with results indicating a higher diversity of VH genes in the bone marrow when compared to the spleen. In addition, presence of shared VH genes between the spleen and bone marrow suggests migration of the V cells between the two compartments. The role of this novel cell population in innate and/or adaptive immunity is currently under investigation. |
| تدمد: | 1550-6606 0022-1767 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::d293e0aa1d9fcd224f0cf4276fdef947 https://doi.org/10.4049/jimmunol.190.supp.52.13 |
| رقم الأكسشن: | edsair.doi...........d293e0aa1d9fcd224f0cf4276fdef947 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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