MicroRNA-151 Attenuates Apoptosis of Endothelial Cells Induced by Oxidized Low-density Lipoprotein by Targeting Interleukin-17A (IL-17A)
| العنوان: | MicroRNA-151 Attenuates Apoptosis of Endothelial Cells Induced by Oxidized Low-density Lipoprotein by Targeting Interleukin-17A (IL-17A) |
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| المؤلفون: | Shou-Liang Miao, Fan-Feng Chen, Guan-Xia Zhu, Xiao-Ning Ye, Hao-Te Jiang |
| المصدر: | Journal of Cardiovascular Translational Research. 14:400-408 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.diagnostic_test, Chemistry, Oxidized low density lipoprotein, Pharmaceutical Science, 030204 cardiovascular system & hematology, Flow cytometry, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Western blot, Downregulation and upregulation, Apoptosis, microRNA, Genetics, Cancer research, medicine, Molecular Medicine, Luciferase, Interleukin 17, Cardiology and Cardiovascular Medicine, Genetics (clinical) |
| الوصف: | Apoptosis of endothelial cells plays an important role in atherosclerosis (AS). MicroRNAs (miRNAs) have been confirmed to participate in the process of endothelial cell apoptosis. The main purpose of this study was to investigate the mechanism of miR-151 and interleukin-17A (IL-17A) in apoptosis of atherosclerotic endothelial cells. The expression levels of miR-151 in human aortic endothelial cells (HAEC) after Ox-LDL treatment were detected by qRT-PCR. The expression levels of IL-17A were detected by qRT-PCR and Western blot. The effects of miR-151 and IL-17A on the apoptosis rate were detected by flow cytometry. The relationship between miR-151 and IL-17A was assessed by bioinformatics analysis and luciferase assay. The expression levels of miR-151 in HAEC after Ox-LDL treatment were reduced, and the expression of IL-17A was upregulated. MiR-151 and si-IL-17A inhibited the apoptosis rate of aortic endothelial cells treated by Ox-LDL. MiR-151 and si-IL-17A reduced the expression levels of c-caspase-9, c-caspase-3, and BAX proteins in Ox-LDL-treated HAEC and increased the expression levels of Bcl-2. MiR-151 inhibited the apoptosis of endothelial cells in AS, and IL-17A was a new target for miR-151. Our findings provided a potential treatment for atherosclerosis in the treatment of AS. |
| تدمد: | 1937-5395 1937-5387 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::d456fe96f7d879bb3f87ec507529eff3 https://doi.org/10.1007/s12265-020-10065-w |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........d456fe96f7d879bb3f87ec507529eff3 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 19375395 19375387 |
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