| الوصف: |
Aim: (1) Simulating vaginal delivery and menopause to establish a mouse model of PGAD; (2) screen out a set of detection methods for quantifying and evaluating mouse sexual function; (3) further explore the relationship and possible mechanism of vaginal microvascular injury and PGAD.Methods: Animal study about established a PGAD mouse model with vaginal dilation and ovariectomized, and ligated vaginal microvascular to compared effects of blood flow on sexual behavior with sham. Animals: C57/B6J mice. Intervention: 8-week-old post-childbirth mice were vaginal dilation and ovariectomy and reared for 1 or 2 months. In addition, ligated microvascular around the vagina to build a vaginal ischemia model.Main Outcome Measures: Video cameras continuously recorded the mice's sexual behavior, vaginal temperature and lubricating were observed by thermometer and water absorption test paper, vagina surface tension was observed by multi-channel physiological recorder. Various stains were used to observe the vaginal morphology, blood vessels, nerves, muscles and fibers of vaginal tissue.Results: (1) Different modeling methods and time will cause atrophy in the vagina and uterus in different groups (F=169.4, p0.05), but the contents of these three hormones decreased after ovariectomy (F=480.9, p0.05). (4) Different modeling methods caused different damage and atrophy in the vaginal tissue, which showed that the thickness of epithelial and muscle layer was reduced, the fibrosis was deepened, the content of muscle, nerve fibers and glycogen were reduced. The above phenomenon was most obvious in the OVX&VD4H group, and the VD2H group was the least. Compared with 1 month, the vaginal tissue had restored at 2 months in most groups. (5) Ovariectomy and vaginal dilation both decreased the number of vaginal microvascular (F=5.4, pConclusion: By simulating different modeling methods and time, we believe that a stable and novel PGAD mouse model can be established after ovariectomy combined with vaginal dilation for 2 hours (OVX&VD2H) and rearing for 1 month. In addition, through a large number of experiments, we screened that sexual behavior, vaginal lubricating, vagina surface tension can be used to detect sexual function in mice objectively and accurately, but vaginal temperature not suitable for the detection of mouse sexual function. Finally, we further verified that microvascular injury can significantly appeared in the model, and the VEGF-ERK signaling pathway is a possible mechanism involved in microvascular injury. |
