Saccharomyces cerevisiaeCells Produce Platelet-activating Factor in Response to Calcium Ionophore A23187
| العنوان: | Saccharomyces cerevisiaeCells Produce Platelet-activating Factor in Response to Calcium Ionophore A23187 |
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| المؤلفون: | Hidehiko Kumagai, Seiji Mitsui, Reiko Nakayama, Hiroaki Udagawa |
| المصدر: | Bioscience, Biotechnology, and Biochemistry. 58:1115-1119 |
| بيانات النشر: | Informa UK Limited, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | Platelet-activating factor, Catabolism, Organic Chemistry, Ionophore, chemistry.chemical_element, General Medicine, respiratory system, Calcium, Biology, Applied Microbiology and Biotechnology, Biochemistry, Analytical Chemistry, EGTA, chemistry.chemical_compound, chemistry, Biosynthesis, Extracellular, lipids (amino acids, peptides, and proteins), PMSF, Molecular Biology, Biotechnology |
| الوصف: | Synthesis of platelet-activating factor (PAF) was stimulated by the treatment of cells with the calcium ionophore A23187 in Saccharomyces cerevisiae. The amount of PAF production increased A23187-concentration- and time-dependently. The maximum PAF synthesis was observed when the yeast cells were treated with 2 μm of A23187 for 5 min, and the PAF amount decreased at the higher concentrations of A23187 and/or for the longer incubation time. Treatment of the cells with 1 mm of PMSF (phenylmethyl-sulfonyl fluoride), an inhibitor of PAF catabolism, resulted in transient accumulation of PAF; however, at more than 2 mm of PMSF, PAF production was reduced. Synthesized PAF was not released into the extracellular fraction and was found to be cell-associated. In the absence of A23187 or CaCl2, and in the presence of EGTA, a Ca2+-chelator, no new synthesis of PAF was observed. These results suggested A23187-induced PAF production was Ca2+ dependent, and proceed via PAF remodeling pathway. PAF synthesis in response t... |
| تدمد: | 1347-6947 0916-8451 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::dadfbea42a1ae9513a7fbd79c58bcf40 https://doi.org/10.1271/bbb.58.1115 |
| رقم الأكسشن: | edsair.doi...........dadfbea42a1ae9513a7fbd79c58bcf40 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 13476947 09168451 |
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