The automation process of Pap smear analysis holds the potential to address women's health care in the face of an increasing population and respective collected data. A fundamental step for automating analysis is cell detection from light microscopy images. Such information serves as input to cell classification algorithms and diagnostic recommendation tools. This paper describes an approach to nuclei cell segmentation, which critically impacts the following steps for cell analyses. We developed an algorithm combining clustering and genetic algorithms to detect image regions with high diagnostic value. A major problem when performing the segmentation of images is the cellular overlay. We introduce a new nuclear targeting approach using heuristics associated with a multi-objective genetic algorithm. Our experiments show results using a public 45-image dataset, including comparison to other cell detection approaches. The findings suggest an improvement in the nuclei segmentation and promise to support more sophisticated schemes for data quality control.
