Continuous expression in tobacco leaves of a Brassica napus PEND homologue blocks differentiation of plastids and development of palisade cells
| العنوان: | Continuous expression in tobacco leaves of a Brassica napus PEND homologue blocks differentiation of plastids and development of palisade cells |
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| المؤلفون: | Paul Wycliffe, Jonny Wernersson, Lars Rask, Mats Ellerström, Folke Sitbon, Ines Ezcurra |
| المصدر: | The Plant Journal. 44:1-15 |
| بيانات النشر: | Wiley, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | biology, Nicotiana tabacum, fungi, food and beverages, Nicotiana benthamiana, Cell Biology, Plant Science, biology.organism_classification, Palisade cell, Cell biology, Chloroplast DNA, Complementary DNA, Botany, Genetics, Cauliflower mosaic virus, Plastid, Plastid envelope |
| الوصف: | Brassica napus complementary deoxyribonucleic acid (cDNA) clones encoding a DNA-binding protein, BnPEND, were isolated by Southwestern screening. A distinctive feature of the protein was a bZIP-like sequence in the amino-terminal portion, which, after expression in Escherichia coli, bound DNA. BnPEND transcripts were present in B. napus roots and flower buds, and to a lesser extent in stems, flowers and young leaves. Treatment in the dark for 72 h markedly increased the amount of BnPEND transcript in leaves of all ages. Sequence comparison showed that BnPEND was similar to a presumed transcription factor from B. napus, GSBF1, a protein deduced from an Arabidopsis thaliana cDNA (BX825084) and the PEND protein from Pisum sativum, believed to anchor the plastid DNA to the envelope early during plastid development. Homology to expressed sequence tag (EST) sequences from additional species suggested that BnPEND homologues are widespread among the angiosperms. Transient expression of BnPEND fused with green fluorescent protein (GFP) in Nicotiana benthamiana epidermal cells showed that BnPEND is a plastid protein, and that the 15 amino acids at the amino-terminal contain information about plastid targeting. Expression of BnPEND in Nicotiana tabacum from the Cauliflower Mosaic Virus 35S promoter gave stable transformants with different extents of white to light-green areas in the leaves, and even albino plants. In the white areas, but not in adjacent green tissue, the development of palisade cells and chloroplasts was disrupted. Our data demonstrate that the BnPEND protein, when over-expressed at an inappropriate stage, functionally blocks the development of plastids and leads to altered leaf anatomy, possibly by preventing the release of plastid DNA from the envelope. |
| تدمد: | 1365-313X 0960-7412 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::df9b5c1c39e1fe93463820305cc21f48 https://doi.org/10.1111/j.1365-313x.2005.02482.x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........df9b5c1c39e1fe93463820305cc21f48 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1365313X 09607412 |
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