Quantitative Bioanalysis Utilizing High-performance Liquid Chromatography/Electrospray Mass Spectrometry via Selected-ion Monitoring of the Sodium Ion Adduct [M + NNa]+
| العنوان: | Quantitative Bioanalysis Utilizing High-performance Liquid Chromatography/Electrospray Mass Spectrometry via Selected-ion Monitoring of the Sodium Ion Adduct [M + NNa]+ |
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| المؤلفون: | Mohammed Jemal, Robert B. Almond, Debbie S. Teitz |
| المصدر: | Rapid Communications in Mass Spectrometry. 11:1083-1088 |
| بيانات النشر: | Wiley, 1997. |
| سنة النشر: | 1997 |
| مصطلحات موضوعية: | Electrospray, Bioanalysis, Chromatography, Sodium, Electrospray ionization, Organic Chemistry, chemistry.chemical_element, High-performance liquid chromatography, Analytical Chemistry, Adduct, chemistry.chemical_compound, chemistry, Selected ion monitoring, Sodium acetate, Spectroscopy |
| الوصف: | A high-performance liquid chromatography (HPLC)/electrospray mass spectrometric method for quantitative determination of a compound in dog plasma was developed and validated via the selected-ion monitoring of the electrospray-generated [M + Na]+ adduct of the compound. The plasma samples were acidified with HCl and then extracted with methyl tert-butyl ether. The reconstituted extracts were injected into an HPLC/positive-ion electrospray ionization mass spectrometry system. The HPLC mobile phase consisted of acetonitrile, water, formic acid (3 mm) and sodium acetate (0.3 mm). This composition of mobile phase provided the optimum electrospray condition for the formation of the [M + Na]+-ion. This work demonstrates that the addition of sodium acetate into the HPLC mobile phase and the subsequent selected-ion monitoring of the sodium ion adduct of the analyte is a viable approach in quantitative bioanalysis. The facile formation of the sodium ion adduct of the analyte, which does not contain functional groups that are known to be strong proton acceptors, appears to be a function of the particular electrospray instrument used. © 1997 John Wiley & Sons, Ltd. |
| تدمد: | 1097-0231 0951-4198 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::dfa4bfb0727a580a87721d6f2821e293 https://doi.org/10.1002/(sici)1097-0231(19970630)11:10<1083::aid-rcm937>3.0.co;2-f |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........dfa4bfb0727a580a87721d6f2821e293 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970231 09514198 |
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