Mutation in afsR Leads to A-Factor Deficiency in Streptomyces griseus B2682
| العنوان: | Mutation in afsR Leads to A-Factor Deficiency in Streptomyces griseus B2682 |
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| المؤلفون: | Zoltán Kele, Dávid Lukács, Zsuzsanna Birkó, Melinda Szilágyi, Sándor Biró, Éva Marton |
| المصدر: | Microbial Physiology. 28:216-224 |
| بيانات النشر: | S. Karger AG, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Genetics, Mutation, Protease, biology, Physiology, medicine.medical_treatment, 030106 microbiology, Mutant, Nonsense mutation, Cell Biology, biology.organism_classification, medicine.disease_cause, Applied Microbiology and Biotechnology, Biochemistry, Microbiology, 03 medical and health sciences, Transformation (genetics), Shuttle vector, medicine, Overproduction, Streptomyces griseus, Biotechnology |
| الوصف: | Background/Aims: A-factor, a γ-butyrolactone autoregulator, in Streptomyces griseus is involved in the regulation of differentiation and antibiotic production. Here we studied the S. griseus B2682-AFN (A-factor negative) bald mutant that harbors a nonsense mutation in the afsR gene encoding a pleiotropic regulator. Our aim was to prove that this mutation is the cause of the A-factor deficiency in AFN. We also studied whether AfsR regulates A-factor production by AfsA, which is supposed to be the only specific key enzyme in A-factor biosynthesis. Methods: Wild afsR was cloned to the pHJL401 shuttle vector and was transformed to the S. griseus AFN and B2682 strains. During phenotypic characterization, sporulation, antibiotic, protease, A-factor, and AfsA protein production were studied. Results: Transformation of AFN by a wild afsR restored its phenotype including sporulation, antibiotic, extracellular protease, and A-factor production. Introduction of afsR to the B2682 wild-type strain resulted in antibiotic and extracellular protease overproduction that was accompanied with an elevated A-factor level. AfsA was detected both in AFN and B2682. Conclusions: AfsR has an effect on the regulation of A-factor production in S. griseus. The presence of AfsA is not sufficient for normal A-factor production. AfsR regulates A-factor biosynthesis independently of AfsA. |
| تدمد: | 2673-1673 2673-1665 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::e02dd87449544cf91c6280f52fabe307 https://doi.org/10.1159/000495410 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........e02dd87449544cf91c6280f52fabe307 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 26731673 26731665 |
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