CRISPR/Cas9-mediated F1534S substitution in the voltage-gated sodium channel reveals its necessity and sufficiency for deltamethrin resistance in Aedes albopictus
| العنوان: | CRISPR/Cas9-mediated F1534S substitution in the voltage-gated sodium channel reveals its necessity and sufficiency for deltamethrin resistance in Aedes albopictus |
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| المؤلفون: | Yijia Guo, Jingni Zhou, Yijie Zhao, Jielin Deng, Xinghua Su, Jianxia Tang, Guoding Zhu, Xiaojie Zhou, Jinbao Gu, Guiyun Yan, Anthony A. James, Xiao-Guang Chen |
| المصدر: | Journal of Pest Science. 96:1173-1186 |
| بيانات النشر: | Springer Science and Business Media LLC, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Ecology, Insect Science, Plant Science, Agronomy and Crop Science, Ecology, Evolution, Behavior and Systematics |
| الوصف: | Insecticide resistance in Aedes mosquitoes presents a major challenge to the control of arboviral diseases. However, resistance mechanisms for many of the insecticides remain unknown. A commonly used insecticide, deltamethrin, was used to select a resistance strain of the vector mosquito, Aedes albopictus, and we identified an F1534S substitution in the voltage-gated sodium channel (VGSC) gene product as the first event in generating resistance. Engineering an F1534S substitution using Cas9/gRNA technologies conferred deltamethrin resistance on a previously susceptible strain. Crosses that removed this mutation restored the susceptible phenotype. Predicted protein structural changes and differences in transcript accumulation levels were correlated with the resistance phenotype. Furthermore, F1534S mutations were detected in all resistant Ae. albopictus populations collected in the field. We conclude that the VGSC F1534S mutation is essential for resistance to deltamethrin in Ae. albopictus, and is a suitable molecular index for pyrethroid resistance detection and monitoring in this species. |
| تدمد: | 1612-4766 1612-4758 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::e25800a3661d05b55cf785109a3edbdd https://doi.org/10.1007/s10340-022-01557-6 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........e25800a3661d05b55cf785109a3edbdd |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 16124766 16124758 |
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