Effects of rosuvastatin on ADMA, rhokinase, NADPH oxidase, caveolin-1, hsp 90 and NFkB levels in a rat model of myocardial ischaemia-reperfusion : cardiovascular topic
| العنوان: | Effects of rosuvastatin on ADMA, rhokinase, NADPH oxidase, caveolin-1, hsp 90 and NFkB levels in a rat model of myocardial ischaemia-reperfusion : cardiovascular topic |
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| المؤلفون: | Necip Ilhan, Oktay Burma, Ayhan Uysal, Deniz Erol, Mete Ozcan, Elif Onat, Engin Sahna |
| المصدر: | Cardiovascular Journal Of Africa. 25:212-216 |
| بيانات النشر: | Clinics Cardive Publishing, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | medicine.medical_specialty, NADPH oxidase, biology, business.industry, nutritional and metabolic diseases, Inflammation, General Medicine, Pharmacology, medicine.disease, medicine.disease_cause, Surgery, Nitric oxide, Rosuvastatin Calcium, chemistry.chemical_compound, chemistry, Caveolin 1, medicine, biology.protein, Rosuvastatin, Endothelial dysfunction, medicine.symptom, Cardiology and Cardiovascular Medicine, business, Oxidative stress, medicine.drug |
| الوصف: | Aim : Endothelial dysfunction, oxidative stress and inflammation are among the most important mechanisms of ischaemia-reperfusion (I/R) injury. Besides their cholesterol-lowering effects, statins are known to provide protection against myocardial dysfunction and vascular endothelial injury via nitric oxide-dependent mechanisms. The aim of this study was to investigate the effects of rosuvastatin on certain intermediates involved in the generation of nitric oxide (asymmetrical dimethyl arginin, ADMA, caveolin-1 and hsp 90), oxidative stress (rhokinase, NADPH oxidase) and inflammation (NFkB), using an in vivo model of myocardial infarction in the rat. Methods : Adult male Sprague Dawley rats were divided into three groups (control, I/R and I/R after 15 days of rosuvastatin administration). Reperfusion was applied for 120 min following left anterior descending coronary artery ischaemia for 30 min. Caveolin-1, hsp 90 and NFkB levels were evaluated with the quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and ADMA, rhokinase and NADPH oxidase levels were evaluated with ELISA. Results : While NFkB and hsp 90 levels were higher in the I/R group, their levels were significantly lower in the rosuvastatin group. While ADMA and NADPH oxidase levels significantly increased with I/R, they were lower in the rosuvastatin-treated group, but not statistically significant. Rhokinase levels were significantly lower in the rosuvastatin group. Caveolin-1 levels were not different between the groups. Conclusion : Our results suggest that ADMA, rhokinase, NADPH oxidase, hsp 90 and NFkB could facilitate I/R injury, and rosuvastatin significantly reduced levels of these parameters. These results indicate that rosuvastatin may have a protective role in I/R injury via mechanisms targeting inflammation, endothelial dysfunction and oxidative stress. |
| تدمد: | 1680-0745 1995-1892 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::e3e4bb2a8822b49df1a4468b6de1cc65 https://doi.org/10.5830/cvja-2014-038 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........e3e4bb2a8822b49df1a4468b6de1cc65 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16800745 19951892 |
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