New vaccination delivery regimen drives enhanced, vaccine-specific immune responses. (155.13)
| العنوان: | New vaccination delivery regimen drives enhanced, vaccine-specific immune responses. (155.13) |
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| المؤلفون: | Rafaella Queiroz, Kelsey Allen, Lisa McEwen, Jennifer Ring, Donald Harn |
| المصدر: | The Journal of Immunology. 186:155.13-155.13 |
| بيانات النشر: | The American Association of Immunologists, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | There is considerable research to define novel and safe adjuvants for use in vaccine formulations. Here, we focused on a gel-slurry delivery method to drive pro-inflammatory vaccine-specific responses using CpGs as adjuvant. The gel slurry hardens at body temperature, forming a gel matrix depot that releases CpGs and antigen, attracting antigen presenting cells. We vaccinated mice with recombinant Hepatitis B antigen using 8 different adjuvant schemes comparing induction of cytokines and antibody subtypes. We compared delivery using two different gel-slurrys with Alhydrogel (Alum) and Complete Freunds adjuvant (CFA). Each was mixed +/- CpGs. Mice vaccinated with gel slurry plus CpGs had significantly higher vaccine-specific antibody responses than mice vaccinated with antigen and CFA. Recall assays showed cells from mice vaccinated with Alhydrogel or CFA upregulated IL-10 at 72h compared to cells from gel-slurry + CpG vaccinated mice. Similar results were seen for IL-4. CpG use reduced levels of IL-5 to background in all groups compared to elevated levels in CFA. No differences in levels of IFNg and TNF. We are currently assaying for Ag-specific antibody isotype titers. The use of gel slurry plus CpGs to deliver vaccine antigens should have great utility for a number of vaccine formulations. Ultimately, this type of vaccine delivery system may facilitate development of therapeutic vaccines as well as prophylactic. |
| تدمد: | 1550-6606 0022-1767 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::e7793b67649f1d4efd3d3af3bb28833d https://doi.org/10.4049/jimmunol.186.supp.155.13 |
| رقم الأكسشن: | edsair.doi...........e7793b67649f1d4efd3d3af3bb28833d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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