Functional and Structural Analyses of trans C-Methyltransferase in Fungal Polyketide Biosynthesis
| العنوان: | Functional and Structural Analyses of trans C-Methyltransferase in Fungal Polyketide Biosynthesis |
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| المؤلفون: | Yuta Tsunematsu, Takuma Matsushita, Yi Tang, Shinji Kishimoto, Kodai Hara, Kenji Watanabe, Hiroshi Hashimoto |
| المصدر: | Biochemistry. 58:3933-3937 |
| بيانات النشر: | American Chemical Society (ACS), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | chemistry.chemical_classification, 0303 health sciences, Chemistry, Stereochemistry, 030302 biochemistry & molecular biology, Mutant, Substrate (chemistry), Methylation, Biochemistry, 03 medical and health sciences, Polyketide, chemistry.chemical_compound, Enzyme, Biosynthesis, Oxidoreductase, Secondary metabolism |
| الوصف: | Biosynthesis of certain fungal polyketide-peptide synthetases involves C-methyltransferase activity that adds one or more S-adenosyl-l-methionine-derived methyl groups to the carbon framework. The previously reported PsoF-MT, the stand-alone C-methyltransferase (MT) from the pseurotin biosynthetic pathway that exists as a domain within a trifunctional didomain enzyme PsoF, was characterized crystallographically and kinetically using mutants with substrate analogs to understand how a trans-acting C-MT works and compare it to known polyketide synthase-associated C-MTs. This study identified key active-site residues involved in catalysis and substrate recognition, which led us to propose the mechanism of C-methylation and substrate specificity determinants in PsoF-MT. |
| تدمد: | 1520-4995 0006-2960 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::e7df32c61915ac49110a168018e13649 https://doi.org/10.1021/acs.biochem.9b00702 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........e7df32c61915ac49110a168018e13649 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15204995 00062960 |
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