Characterization of Pseudomonas syringae pv. syringae from Diseased Stone Fruits in Kyrgyzstan and Testing of Biological Agents against Pathogen
| العنوان: | Characterization of Pseudomonas syringae pv. syringae from Diseased Stone Fruits in Kyrgyzstan and Testing of Biological Agents against Pathogen |
|---|---|
| المؤلفون: | Saikal Bobusheva, Tinatin Doolotkeldieva |
| المصدر: | International Journal of Phytopathology. 9:71-91 |
| بيانات النشر: | EScience Press, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Prunus salicina, biology, Plant Science, Erwinia, biology.organism_classification, 01 natural sciences, Agricultural and Biological Sciences (miscellaneous), Prunus armeniaca, Microbiology, 03 medical and health sciences, Prunus, 030104 developmental biology, Xanthomonas, Pseudomonas syringae, Stenotrophomonas, Agronomy and Crop Science, Pathogen, 010606 plant biology & botany |
| الوصف: | The plant diseases caused by the Pseudomonas syringae сomplex b acteria are economically important and occur worldwide on various plants, and it is as a pathogen that has not been the object of studies and little is known about its epidemiology in Kyrgyzstan. The conventional phenotypic ( LOPAT, API tests) and PCR-assisted isolation were used for the identificationof Pseudomonas syringae pv. syringaе isolates from the affected organs of local stone fruits, such as peach ( Prunus persica ), cherry ( Prunus subgen ), apricot ( Prunus armeniaca), and plum ( Prunus salicina ) samples taken from the Chy, Issuk-Kul, and Batken regions of the country. 16S rRNA gene a mplification was performed with primers 27F (5'-AGA GTT TGA TCC TGG CTC AG -3') and 907R (5 '–CCG TCA ATT CCT TTG AGT TT-3') for the identification of obtained P.syringae pv. syringaе isolates. From 40 primary isolates of Gram -negative rod-shaped bacteria, 1 2 were identified as Pseudomonas syringae pv. syringae, while the remaining isolates were identified as bacteria from Stenotrophomonas , Xanthomonas, Erwinia genera . The antagonist bio control agent— Streptomyces b acteria strains were screened and selected against the bacterial canker pathogen in in vitro experiments and on apricot seedlings in vivo conditions. Obtained results could encourage to develop a local bio-product based on this bioagent for spraying stone fruits with the initial manifestation of disease symptoms and to conduct preventive treatments in the fall and spring to increase the plant's resistance to pathogens. |
| تدمد: | 2312-9344 2313-1241 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::e9dd121aa5c361fdc7e3f8371e861790 https://doi.org/10.33687/phytopath.009.02.3270 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........e9dd121aa5c361fdc7e3f8371e861790 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23129344 23131241 |
|---|