P-228 Higher oxidation levels in follicular fluid correlate with better outcome in ICSI treatments
| العنوان: | P-228 Higher oxidation levels in follicular fluid correlate with better outcome in ICSI treatments |
|---|---|
| المؤلفون: | J.M De Los Santos, M.Á Valera, F Meseguer, L Alegre, E Paya, P Gámiz, M Meseguer |
| المصدر: | Human Reproduction. 37 |
| بيانات النشر: | Oxford University Press (OUP), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Reproductive Medicine, Rehabilitation, Obstetrics and Gynecology |
| الوصف: | Study question Can the oxidative status of the follicular fluid be a valid biomarker of the quality of the oocyte cohort and clinical outcome in ICSI treatments? Summary answer The follicular fluid (FF) of oocyte cohorts that achieved at least one pregnancy, ongoing pregnancy and life birth have higher oxidative stress levels. What is known already The role of oxidative stress in female infertility is yet unclear, due to the numerous factors that participate in the production and activity of reactive oxygen species (ROS), and the different techniques used for its detection. The Fertissimo TCL AnalyzerTM (Carmel Diagnostics, Israel) uses thermochemiluminiscence to assess the oxidative status (OS) of biological samples by counting the photons emitted per second (cps) after a heat-catalyzed oxidative reaction. Using this technique, a negative correlation was found between ROS levels in FF and patient age (Wiener-Megnazi et al, 2004), but its potential as a biomarker of clinical outcome is unclear. Study design, size, duration Prospective observational study performed in a single IVF center during one year. 173 women were subjected to controlled ovarian stimulation as part of an autologous (103 patients) or ovum donation ICSI treatment (70 donors). The OS of the follicular fluid (FF) obtained in the oocyte retrieval was assessed and compared with the characteristics of the treatment and clinical outcome. Participants/materials, setting, methods The first tube of follicular fluid obtained in the oocyte retrieval was centrifuged (10 minutes at 600g) and the supernatant was analyzed in the Fertissimo TCL AnalyzerTM. OS results were normalized per volume and summarized as TCL-Score (average photon counting from 50-280s) and Ratio (slope of the reaction time curve). TCL parameters were compared with the demographics, number of oocytes retrieved and clinical outcome of the cohort using Pearsons Correlation and Anova tests Main results and the role of chance FF from donors had higher TCL-Score (44.27±14.41cps vs 40.59±11.65cps, P = 0.066) and Ratio (367.38±89.47 vs 329.88±78.12, P = 0.004) than patients. A significant inverse correlation was found between TCL Ratio and the age of the women (Pearsons Correlation Coefficient (PCC)=-0.207; P = 0.006). A direct significant correlation was found between the body mass index (BMI) and the TCL-Score (PCC=0.186, P = 0.014) and Ratio (PCC=0.186, P = 0.014). TCL-Score (PCC=0.173, P = 0.023) and Ratio (PCC= 0.275, P Limitations, reasons for caution Some miscalibration issues were met in the course of the analysis, which may hinder its reproducibility. Stimulation protocol or sperm quality was not considered in the analysis. A larger scale, controlled trial should be performed to validate results. Wider implications of the findings Presented results are in tune with previously published data using similar and different techniques for assessing oxidative and antioxidative capacity in FF samples. The analysis of the oxidative status of the FF could be a valid biomarker of clinical outcome of ICSI treatments and as indicator for antioxidant therapy. Trial registration number APOTIP/2020/015 Generalitat Valenciana. |
| تدمد: | 1460-2350 0268-1161 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::edd6c980f0e81e2197ed7747cfc79633 https://doi.org/10.1093/humrep/deac107.220 |
| حقوق: | EMBARGO |
| رقم الأكسشن: | edsair.doi...........edd6c980f0e81e2197ed7747cfc79633 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602350 02681161 |
|---|