IDENTIFICATION OF POTEXVIRUS ISOLATES FROM CREEPING PHLOX AND TRAILING PORTULACA AS STRAINS OF ALTERNANTHERA MOSAIC VIRUS, AND COMPARISON OF THE 3´-TERMINAL PORTION OF THE VIRAL GENOMES
| العنوان: | IDENTIFICATION OF POTEXVIRUS ISOLATES FROM CREEPING PHLOX AND TRAILING PORTULACA AS STRAINS OF ALTERNANTHERA MOSAIC VIRUS, AND COMPARISON OF THE 3´-TERMINAL PORTION OF THE VIRAL GENOMES |
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| المؤلفون: | John Hammond, C. J. Maroon-Lango, M. D. Reinsel |
| المصدر: | Acta Horticulturae. :71-78 |
| بيانات النشر: | International Society for Horticultural Science (ISHS), 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Antiserum, Phlox stolonifera, biology, Alternanthera mosaic virus, Portulaca grandiflora, Phlox, Botany, Horticulture, Portulaca, biology.organism_classification, Potexvirus, Virology, Papaya mosaic virus |
| الوصف: | Potexvirus isolates from creeping phlox (Phlox stolonifera) and trailing portulaca (Portulaca grandiflora) obtained from commercial nurseries were found to react strongly with polyclonal antiserum against Papaya mosaic virus (PapMV). Cloning and sequencing of a phlox isolate from Pennsylvania revealed that the coat protein (CP) gene was c.97% identical at the amino acid level to the CP gene sequence of Alternanthera mosaic virus (Geering and Thomas, 1999), and only c.75% to the CP of PapMV. The full genomic sequence of the phlox isolate was then determined. The regions to the 5' of the CP gene were found to be more closely related to (but distinct from) PapMV than to any other characterized potexvirus. This phlox potexvirus isolate was therefore named AltMV-PA. The host range of AltMV-PA was found to be similar to that of the Australian isolate, AltMV-Au. The 3'-terminal portion of the genome of the portulaca potexvirus isolate, and of additional potexvirus isolates from two phlox cultivars from commercial nurseries in Maryland, were also cloned and sequenced. The sequences of these isolates were similar to those of AltMV-Au and AltMV-PA, and the isolates were designated AltMV-Po (Portulaca), AltMV-SP (creeping phlox 'Sherwood Purple'), and AltMV-BR (creeping phlox 'Blue Ridge'). Each of the phlox and portulaca isolates reacted strongly with antiserum to AltMV-Au, and also with anti-PapMV antiserum. The host range of AltMV includes several ornamentals; these findings suggest that AltMV may be quite widespread, and may be mis-diagnosed as PapMV. |
| تدمد: | 2406-6168 0567-7572 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::ee799baceba91d8acb4ea1aa29b4302c https://doi.org/10.17660/actahortic.2006.722.8 |
| رقم الأكسشن: | edsair.doi...........ee799baceba91d8acb4ea1aa29b4302c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 24066168 05677572 |
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