Mitochondrial peroxiredoxin-3 protects hippocampal neurons from excitotoxic injury in vivo
| العنوان: | Mitochondrial peroxiredoxin-3 protects hippocampal neurons from excitotoxic injury in vivo |
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| المؤلفون: | Shinzo Oikawa, Takafumi Noshita, Fumiyuki Hattori, Norihito Murayama |
| المصدر: | Journal of Neurochemistry. 86:860-868 |
| بيانات النشر: | Wiley, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Programmed cell death, Peroxiredoxin III, Neurotoxicity, Oxidative phosphorylation, Mitochondrion, Biology, medicine.disease, Biochemistry, Neuroprotection, Cell biology, Cellular and Molecular Neuroscience, medicine, Neurotoxin, Peroxiredoxin |
| الوصف: | Mitochondria are involved in excitotoxic damage of nerve cells. Following the breakdown of the calcium-buffering ability of mitochondria, mitochondrial calcium overload induces reactive oxygen species (ROS) bursts that produce free radicals and open permeability transition pores, ultimately leading to neuronal cell death. In the present study, we focused on a mitochondrial antioxidant protein, peroxiredoxin-3 (Prx-3), to investigate the mechanism by which toxic properties of ROS were up-regulated in mitochondria of damaged nerve cells. Immunohistochemical analysis revealed that Prx-3 protein exists in mitochondria of rat hippocampus, whereas we found a significant decrease in Prx-3 mRNA and protein levels associated with an increase in nitrated proteins in the rat hippocampus injured by microinjection of ibotenic acid. Furthermore, in vivo adenoviral gene transfer of Prx-3 completely inhibited protein nitration and markedly reduced gliosis, a post-neuronal cell death event. Since mitochondrial Prx-3 seems to be neuroprotective against oxidative insults, our findings suggest that Prx-3 up-regulation might be a useful novel approach for the management of neurodegenerative diseases. |
| تدمد: | 1471-4159 0022-3042 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::eea5e38c0e8cb798f70f8b232343a8d4 https://doi.org/10.1046/j.1471-4159.2003.01918.x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........eea5e38c0e8cb798f70f8b232343a8d4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14714159 00223042 |
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