The southern sandshell, Lampsilis australisSimpson, 1900, is a rare freshwater mussel endemic to the Escambia, Yellow, andChoctawhatchee river drainages in Alabama and Florida. Laboratory observationsconfirmed L. australis as a superconglutinate producer.Superconglutinate and glochidial morphology are similar to those of othersuperconglutinate producers. The current distribution of L.australis, as determined from recent surveys (1995–2000) andfield notes (1990–2000), is compared with its historical distribution asdefined by museum collections and field notes made between 1900 and 1989. Thecurrent distribution of L. australis is reduced by76% compared to its historical distribution, although L. australis waslocated at new sites in the recent surveys. Relative abundance of L.australis was low at all but two sites. Based on reproductivestrategy, range reductions, low relative abundance, and potential threats withinthe Escambia, Yellow, and Choctawhatchee river drainages, we believeL. australis should be considered for protection under theEndangered Species Act.