In vitro cytotoxicity of biosynthesized titanium dioxide nanoparticles in human prostate cancer cell lines
| العنوان: | In vitro cytotoxicity of biosynthesized titanium dioxide nanoparticles in human prostate cancer cell lines |
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| المؤلفون: | Xiaosong Fan, Baiye Jin, Feiping He, Weixing Yu |
| المصدر: | Tropical Journal of Pharmaceutical Research. 16:2793 |
| بيانات النشر: | African Journals Online (AJOL), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Aqueous solution, food.ingredient, technology, industry, and agriculture, Pharmaceutical Science, Nanoparticle, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, chemistry.chemical_compound, food, Dynamic light scattering, chemistry, Titanium dioxide, Zeta potential, Pharmacology (medical), Fourier transform infrared spectroscopy, 0210 nano-technology, Cytotoxicity, Cinnamomum tamala, Nuclear chemistry |
| الوصف: | Purpose: To establish a green method for production of titanium dioxide (TiO 2 ) nanoparticles (NPs) using Cinnamomum tamala (C. tamala) leaf extract, and examine the in vitro cytotoxicity of the product in a human prostate cancer (D145) cell line. Methods: TiO 2 NPs were synthesized by mixing 20 mL of C. tamala leaf extract with 0.1 M titanium dioxide (Ti(OH) 2 ) (80 mL) in aqueous solution with stirring for 2 h at room temperature. The TiO 2 NPs were characterized using x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), x-ray photoelectron spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM), selected-area electron diffraction, and energy dispersive x-ray spectroscopy. The in vitro cytotoxicity against D145 cells was determined using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Results: TEM and DLS analyses showed that the NPs were irregularly shaped, with an average particle size of 23 nm. The FT-IR spectrum of C. tamala leaf extract showed that the biomolecules were potentially involved in reduction processes. The negative zeta potential of -14 mV indicated that the NPs were stable and discrete while their crystalline nature was confirmed by XRD. Cytotoxicity analysis showed that the TiO 2 NPs exhibit a dose-dependent toxic effect on D145 cells. Conclusion: A facile and less expensive approach for the production of TiO 2 NPs using C. tamala leaf extract has been developed. The TiO 2 NPs showed dose-dependent cytotoxicity towards D145 cells. Keywords : Anticancer activity, Cinnamomum tamala, Green synthesis, Prostate cancer, TiO 2 nanoparticles |
| تدمد: | 1596-9827 1596-5996 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::f2880b36609814ac07319dc6802be179 https://doi.org/10.4314/tjpr.v16i12.2 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........f2880b36609814ac07319dc6802be179 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15969827 15965996 |
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